SbtA | Sodium-dependent bicarbonate transporter
AS13 2657 | Clonality: Polyclonal | Host: Rabbit | Reactivity: cyanobacteria
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|Recommended dilution||1 : 2000 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Cyanobium sp. PCC7001, Synechochoccus elongatus sp.PCC7942, Synechochoccus sp. PCC7002, Synechocystis sp. PCC6803|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
SbtA from Cyanobium PCC7001 is expected to be smaller on SDS-PAGE.
|Selected references||Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.
Holland et al. (2016). Impacts of genetically engineered alterations in carbon sink pathways on photosynthetic performance. Algal Research, 20 (2016) 87–99.
Negative control, SbtA deletion strain (1), Synechochoccus elongatus PCC7942 (2,3), Cyanobium sp PCC7001 (4), Synechochoccus sp PCC7002 (5), Synechocystis sp PCC6803 (6) extracted with were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed using ATTOPhos.
Courtesy of Dr. Britta Forster, The Australian National University, Canberra, Australia
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