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SbtA | Sodium-dependent bicarbonate transporter

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AS13 2657  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: cyanobacteria

PRODUCT INFORMATION IN PDF

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AS13 2657

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product information
Background

SbtA (Sodium-dependent bicarbonate transporter)

Immunogen

KLH-conjugated synthetic peptide derived from known cyanobacterial SbtaA sequences including Q5MZ69

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized
Quantity 50 µg
Reconstitution Please add 50 µl of sterile water For reconstitution
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products
Additional information
application information
Recommended dilution

1 : 2000 with standard ECL (WB)

Expected | apparent MW

39 kDa

Confirmed reactivity

Cyanobium sp PCC7001, Synechochoccus elongatus PCC7942, Synechochoccus sp PCC7002, Synechocystis sp PCC6803

Predicted reactivity

cyanobacteria

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

SbtA from Cyanobium PCC7001 is expected to be smaller on SDS-PAGE.

Selected references
Holland et al. (2016). Impacts of genetically engineered alterations in carbon sink pathways on photosynthetic performance. Algal Research, 20 (2016) 87–99.

Application example

western blot using anti-SbtA antibodies

Negative control, SbtA deletion strain (1), Synechochoccus elongatus PCC7942 (2,3), Cyanobium sp PCC7001 (4), Synechochoccus sp PCC7002 (5), Synechocystis sp PCC6803  (6) extracted with   were separated on 12  % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with   for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed using ATTOPhos.

Courtesy of Dr. Britta Forster, The Australian National University,  Canberra, Australia


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