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U1 snRNP protein A | U1 small nuclear ribonucleoprotein A

265 €
Buy 2 items of this product for 198 €/each
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AS14 2777 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

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Item No:
AS14 2777

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product information
Background

U1 snRNP protein A (U1 small nuclear ribonucleoprotein A) is involved in mRNA splicing by spliceosome where it is associated with snRNP U1. Involved  in response to salt stress.

Immunogen

KLH-conjugated peptide, derived from Arabidopsis thaliana U1 snRNP, UniProt: Q39244, TAIR: At2g47580

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized
Quantity 50 µg
Reconstitution

For reconstitution add 50 µl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

Western blot (WB)

Related products

Collection of antibodies to micro RNA

Collection of antibodies to proteins involved in salt stress response

Recommended secondary antibody for ECL detection

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 2000 (WB)

Expected | apparent MW

28 | 35 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

 

Not reactive in

 

Additional information
Selected references To be added when available, antibody released in February 2017.

Application example

western blot using anti-U1 snRNP protein A | U1 small nuclear ribonucleoprotein A antibodies


Total protein from rosette leaves> 30 ug of Arabidopsis thaliana whole leaf extract (wild-type) (1), 30 ug of Arabidopsis thaliana whole leaf extract (u1a) (2), extracted with buffer (100mM Tris, 10% glycerol, 5mM EDTA, 5mM EGTA, 0.15M NaCl, 0.75% Triton X100, 0.05% SDS, 1mM DTT) and denatured with 0.06M Tris-HCl, 5% glycerol, 2% SDS, 4% β-mercaptoethanol, 0.0025% bromophenol blue at 95 C for 5 min were separated on 14 % SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 5% low-fat dried milk in TBS + 0,1%Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation in 2% low-fat dried milk in TBS + 0,1%Tween. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:10 000 in 2% low-fat dried milk in TBS + 0,1%Tween for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL Prime, GE Healthcare. Exposure time was 60 seconds.
MW markers: PageRuler Prestained (ThermoFisher)

Courtesy of Agata Stępień, Adam Mickiewicz University, Poland

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