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UBQ11 | Ubiquitin (affinity purified)

272 €
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AS08 307A  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, M. domestica, N. benthamina, O. sativa

 

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AS08 307A

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product information
Background

Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).

Immunogen

Recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal His-tag; TAIR:  At4g05050

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products
AS08 307 | UBQ11 | Ubiquitin, rabbit antibody, provided in a serum format

AS10 307S | UBQ11 | Ubiquitin protein | positive control
Additional information
application information
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

8.5 kDa

Confirmed reactivity Arabidopsis thaliana, Malus domestica, Nicotiana benthamina, Oryza sativa
Predicted reactivity Brachypodium distachyon, Brassica napus, Capsella rubella, Cochliobolus heterostrophus, Citrus clementina, Citrus sinensis, Coffea canephora, Cymbidium faberi, Eucalyptus grandis, Erythrane guttata, Glycine max, Glycine soja, Ipomoea batatas, Medicago truncatula, Magnaporthe oryzae, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Populus trichocarpa, Prunus persica, Pyrus communis, Ricinus communis, Solanum nigrum, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Theobroma cacao, Zea mays , Vitis vinifera
Not reactive in Algae
Additional information

Antibody can be used to check qubiquitination status in the whole plant extracts.

Selected references Krasuska et al. (2016). Nitric oxide-polyamines cross-talk during dormancy release and germination of apple embryos. Nitric Oxide. 2016 Nov 23. pii: S1089-8603(16)30178-1. doi: 10.1016/j.niox.2016.11.003. [Epub ahead of print]

Application example


 western blot using affinity purified anti plant ubiquitin antibody

10 μl of a total protein from Arabidopsis thaliana wt (20 μg of total protein) and Nicotiana benthamiana, was separated on 8% polyacralymide gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1: 5 000 anti-ubiquitin antibody (3h), followed by incubation with 1: 10 000 secondary anti-rabbit antibodies (1h) coupled with HRP and visualization (1 minute exposure) with standard ECL.


Technical note: It is very difficult to detect ubiquitin monomers in total cell extracts due to a great abundance of poly and multi-ubiquitinated proteins. Recommended is size separation of protein extracts before gel electrophoresis focused on good resolution of region between 6-10 kDa. 

Courtesy of Dr. Ari Sadanandom, University of Durham, UK


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com