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UBQ11 | Ubiquitin, affinity purified antibody

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS08 307A  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, M. domestica, N. benthamina, O. sativa

 

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AS08 307A

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product information
Background

Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).

Immunogen

Recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal His-tag; TAIR:  At4g05050

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized
Quantity 50 µg
Reconstitution
For reconstitution add 50 µl of sterile water
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products
AS08 307 | UBQ11 | Ubiquitin, rabbit antibody, provided in a serum format

AS10 307S | UBQ11 | Ubiquitin protein | positive control
Additional information
application information
Recommended dilution

1: 10 000 with standard ECL (WB)

Expected | apparent MW

8.5 kDa

Confirmed reactivity Arabidopsis thaliana, Malus domestica, Nicotiana benthamina, Oryza sativa
Predicted reactivity

dicots including: Nicotiana tabacum, Glycine max, Pisum sativum, monocots including: Hordeum vulgare, Oryza sativa, Zea mays  

Not reactive in

algae

Additional information

Antibody can be used to check qubiquitination status in the whole plant extracts.

Selected references Krasuska et al. (2016). Nitric oxide-polyamines cross-talk during dormancy release and germination of apple embryos. Nitric Oxide. 2016 Nov 23. pii: S1089-8603(16)30178-1. doi: 10.1016/j.niox.2016.11.003. [Epub ahead of print]

Application example


 western blot using affinity purified anti plant ubiquitin antibody

10 μl of a total protein from Arabidopsis thaliana wt (20 μg of total protein) and Nicotiana benthamiana, was separated on 8% polyacralymide gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1: 5 000 anti-ubiquitin antibody (3h), followed by incubation with 1: 10 000 secondary anti-rabbit antibodies (1h) coupled with HRP and visualization (1 minute exposure) with standard ECL.


Technical note: It is very difficult to detect ubiquitin monomers in total cell extracts due to a great abundance of poly and multi-ubiquitinated proteins. Recommended is size separation of protein extracts before gel electrophoresis focused on good resolution of region between 6-10 kDa. 

Courtesy of Dr. Ari Sadanandom, University of Durham, UK


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