XLG2 | Extra-large G-protein 2

Samples:
Left panel: 50 ng of recombinant AtXLG2 protein
Right panel: 20 µg of a total protein from Arabidopsis thaliana wt (wt) and xlg1,2,3 goa1-3 quadruple mutant (mut) 

Samples were separated on 12 % SDS-PAGE  and blotted for 1h to PVDF using wet transfer. Blot was blocked with 5 % milk for 1h/RT. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1h/4°C with agitation in TBS-T. The antibody solution was decanted, and the blot was washed. Blot was incubated in a matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) for 1h/RT with agitation. The blot was washed and developed with chemiluminescence detection reagent. 

Courtesy of Dr. Alan M. Jones, University of North Carolina, USA