Can tubulin be used as a loading control in metal stress? To check this, Anti-tubulin alpha chain antibodies were used on extracts from Nicotiana tabacum plants, treated with silver nanoparticles (AgNPs) with 2 different coatings. Tubulin alpha protein content (marked by a blue arrow in the image to the right) was influenced by silver nanoparticle treatment. Similar results were obtained for tubulin gamma chain. Therefore, it can be concluded that: Metal induced stress affected the expression of tubulin alpha and gamma, and that therefore, these proteins cannot be used as loading controls under these conditions. | All experimental details can be found here. Courtesy of MSc, Karla Košpić, University of Zagreb, Faculty of Science Department of Biology, Croatia. |
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