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product information
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| background |
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Vitellogen (VTG) is a glucolipoprotein yolk precursor produced by all oviparous animals. This protein is female-specific and its expression is under hormonal control (estrogen). However, in the presence of Endocrine Disrupting Chemicals (EDC's), males can express the VgTG gene in a dose dependent manner. The use of VTG gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDC's. |
| immunogen |
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native vitellogenin purified from plasma of estradiol induced male of Senegalese sole (Solea senegalensis) |
| antibody format |
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rabbit; |
polyclonal; |
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serum; |
lyophilized |
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| quantity |
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200 µl |
- for reconstitution add 200 µl of sterile water |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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Western blot (WB), ELISA (ELISA) |
| related products |
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collection of antibodies to fish proteins |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 5 000 (WB), 1: 5 000 on sole serum (ELISA) |
| expected | apparent MW |
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ca. 200 kDa |
| confirmed reactivity |
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Senegalese sole, Dicentrarchus labrax (sea bass), Sparus aurata (seabream) |
| predicted reactivity |
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n. a. |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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the developed VTG ELISA, using these VTG and AbVTG, has been validated for Senegalese sole, sea bass (Dicentrarchus labrax) and seabream (Sparus aurata), which all gave parallel displacement curves in the assay. Probably, plasmas from several other fish species displace parallel and can also be used in the assay, although it has to be validated for each case. |
| selected references |
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Guzman et al. (2008) Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis).Gen and Comp Endocrinology 156: 285-297. |
application example 
STD: Protein standard (molecular weights of the proteins are indicated on the left) (1) plasma from male sole (load 0.11 ul of plasma),(2) plasma from vitellogenic female sole (load 0.11 ul of plasma), (3) plasma from estradiol-treated male sole (load 0.08 ul of plasma,(4) VTG precipitate (load 0.83 ul of precipitate),(5) purified VTG (load 2 ul of purified preparation, corresponding to around 0.14 ug and spawning performance in cultured VTG) were separated on SDS-PAGE 7.5% resolving gel, 4% stacking gel. Samples were denatured in SDS and b- mercaptoethanol and treated 4 min 95 oC before loading. Following gel electrophoresis proteinswere transfered to PVDF Membrane (Inmobilon-P, Millipore) for 2 h. Blots were blocked in TBST containing 2% non-fat dry milk. Blots were incubated in primary antibody at a dilution 1: 40 000, followed by incubation with secondary antibodies (GAR-HRP, BioRad, dilution 1:2,000) and chemiluminiscence development (Luminol Reagent, Santa Cruz Biotechnology).
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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