ATG4 | Autophagy protein 4
AS15 2831 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii
|Info:||More information||Product suggestions||Add review|
|Expected | apparent MW||
64.2 | 70 kDa
|Predicted reactivity||Arabidopsis thaliana, Coccomyxa subellipsoidea C-169, Tetraselmis sp. GSL018, Triticum aestivum, Volvox carteri|
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
|Additional information||to be added when available|
|Selected references||to be added when available, antibody released in July 2015|
10 ng of recombinant, purified Chlamydomonas reinhardtii ATG4 (left) and 15 µg of Chlamydomonas reinhardtii total cell extract (right) were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry transfer. Blots were blocked with 5 % dry milk in PBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5000 over night at 4 ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:10000 in 5 % dry milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Luminata Crescendo (Millipore) according to the manufacturer's instructions. Exposure time was 30 seconds.
Courtesy of María Esther Pérez-Pérez and Jose L. Crespo.
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