ATG4 | Autophagy protein 4

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AS15 2831 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii


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Item No:
AS15 2831

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product information
Background ATG4 (Autophagy protein) is a protein with cyteine-type endopeptidase activity involved in autophagy process. 
Immunogen recombiant ATG4 of Chlamydomonas reinhardtii, Gene ID Cre12.g510100
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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Plant and algal protein extraction buffer

Secondary antibodies

Additional information

this antibody is recognizing 25 ng of recombinant CrATG4

application information
Recommended dilution

1:5000 (WB)

Expected | apparent MW

64.2 | 70 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity Arabidopsis thaliana, Coccomyxa subellipsoidea C-169, Tetraselmis sp. GSL018, Triticum aestivum, Volvox carteri
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information to be added when available
Selected references to be added when available, antibody released in July 2015

application example

western blot using anti-ATG4 antibodies

10 ng of recombinant, purified Chlamydomonas reinhardtii ATG4 (left) and 15 µg of Chlamydomonas reinhardtii total cell extract (right) were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry transfer. Blots were blocked with 5 % dry milk in PBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5000 over night at 4 ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:10000 in 5 % dry milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Luminata Crescendo (Millipore) according to the manufacturer's instructions. Exposure time was 30 seconds.

Courtesy of María Esther Pérez-Pérez and Jose L. Crespo.

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