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ATG4 | Autophagy protein 4

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS15 2831  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

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AS15 2831

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product information
Background
ATG4 (Autophagy protein 4) is a protein with cyteine-type endopeptidase activity involved in autophagy process.
Immunogen recombiant ATG4 of Chlamydomonas reinhardtii, Gene ID Cre12.g510100
Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
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Plant and algal protein extraction buffer

Secondary antibodies

Additional information

This antibody is recognizing 25 ng of recombinant CrATG4

application information
Recommended dilution

1:5000 (WB)

Expected | apparent MW

64.2 | 70 kDa

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity
Coccomyxa subellipsoidea C-169, Tetraselmis sp. GSL018, Triticum aestivum, Volvox carteri
Not reactive in Arabidopsis thaliana
Additional information
Technical details how to work with this antibody are provided here: Pérez-Pérez et al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.
Selected references Pérez-Pérez et al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.
Chen et al. (2016). The role of nitric oxide signalling in response to salt stress in Chlamydomonas reinhardtii. Planta. 2016 Sep;244(3):651-69. doi: 10.1007/s00425-016-2528-0. Epub 2016 Apr 26.

Application example

western blot using anti-ATG4 antibodies
10 ng of recombinant, purified Chlamydomonas reinhardtii ATG4 (left) and 15 µg of Chlamydomonas reinhardtii total cell extract (right) were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry transfer. Blots were blocked with 5 % dry milk in PBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5000 over night at 4 ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:10000 in 5 % dry milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Luminata Crescendo (Millipore) according to the manufacturer's instructions. Exposure time was 30 seconds

Courtesy of María Esther Pérez-Pérez and Jose L. Crespo.

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com