Cat | Catalase (peroxisomal marker)
AS09 501 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, B. napus, Ho. vulgare, N. bentamina, N.tabacum, O.sativa, Z.mays
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|Recommended dilution||2 ĩg (IP), 1 : 1000 (WB)|
|Expected | apparent MW||
57 | 55 kDa
|Confirmed reactivity||Arabidopsis thaliana, Aponogeton madagascariensis, Brassica oleracea, Hordeum vulgare, Nicotiana bentamina, Nicotiana tabacum, Oryza sativa, Paulownia tomentosa, Solanum lycopersicum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera|
Avicennia marina, Betula pendula, Brachypodium distachyon, Brassica napus, Brassica rapa subsp. pekinensis, Brassica campestris, Citrus sp., Citrus maxima, Citrus clementina, Camellia sinensis, Cucumis sativus, Elaeis guineensis var. tenera, Eucalyptus grandis, Fragaria ananassa, Glycine max, Gossypium mexicanum, Litchi chinensis, Hibiscus cannabinus, Saccharum officinarum, Cucurbita maxima, Cucurbita moschata, Morus notabilis, Nicotiana tabacum, Pinus pinea, Populus jackii, Prunus persica, Raphanus sativus, Sesamum indicum, Paenibacillus sp.
|Not reactive in||
To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details.
|Selected references||Zhang et al. (2017). Global analysis of protein lysine succinylation profiles in common wheat. BMC Genomics. 2017 Apr 20;18(1):309. doi: 10.1186/s12864-017-3698-2. (Triticum aestivum L., immunoprecipitation)
Kneeshaw et al. (2017). Nucleoredoxin guards against oxidative stress by protecting antioxidant enzymes. Proc Natl Acad Sci U S A. 2017 Jul 19. pii: 201703344. doi: 10.1073/pnas.1703344114.
Dauphinee et al. (2017). Remodelling of lace plant leaves: antioxidants and ROS are key regulators of programmed cell death. Planta. 2017 Apr 7. doi: 10.1007/s00425-017-2683-y. (Aponogeton madagascariensis)
Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.
Lee et al. (2016). Superoxide serves as a putative signal molecule for plant cell division: overexpression of CaRLK1 promotes the plant cell cycle via accumulation of O2 − and decrease in H2O2. Physiol Plantarum. doi:10.1111/ppl.12487
Kataya et al. (2015). Protein Phosphatase 2A Holoenzyme Is Targeted to Peroxisomes by Piggybacking and Positively Affects Peroxisomal β-Oxidation. Plant Physiol. 2015 Feb;167(2):493-506. doi: 10.1104/pp.114.254409.
Pilati et al. (2014). The onset of grapevine berry ripening is characterized by ROS accumulation and lipoxygenase-mediated membrane peroxidation in the skin. BMC Plant Biol. 2014 Apr 2;14(1):87.
Blume et al. (2013).A possible role for the chloroplast pyruvate dehydrogenase complex in plant glycolate and glyoxylate metabolism. Phytochemistry Aug2.
Li et al. (2013). LESION SIMULATING DISEASE1 Interacts with Catalases to Regulate Hypersensitive Cell Death in Arabidopsis. Plant Physiol. Aug 19.
Marok et al. (2013). A drought-sensitive barley variety displays oxidative stress and strongly increased contents in low-molecular weight antioxidant compounds during water deficit compared to a tolerant variety. J. Plant Physioology, Feb 8.
10 µg of total protein from Arabidopsis thaliana Col0 (1), Cat2-(Col0) (2), Ler0 (3), Cat2-(Ler0) (4), Zea mays (5), Oryza sativa (6), Brassica oleracea (7), Nicotiana bentamina (8) were extracted with 60mM Tris pH 6.9, 10mM DTT, 20% glycerol, 1mm PMSF were separated on 12.5% SDS-PAGE and blotted 1h to PVDF. Blot was blocked with 3% skim milk in PBS+0.05% Tween20 for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in the same buffer. The antibody solution was decanted and the blot was rinsed briefly three times, then washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera, AS09 602) diluted to 1:50 000 in 3% skim milk in PBS+0.05% Tween20 for 1h at RT with agitation. The blot was washed as above and developed for 1 min with Western Lightning Plus-ECL ( PerkinElmer )according to the manufacturers instructions. Exposure time was 5min. in ChemiDoc XRS+ (Biorad ).
Courtesy of Brigitte van de Cotte, Gent University, Belgium
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