EF-G1 | elongation factor G1
AS13 2652 | clonality: polyclonal | host: rabbit | reactivity: Synechocystis sp. PCC6803
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1: 4000 with standard ECL (WB)
|Expected | apparent MW||
|Confirmed reactivity||Synechocystis sp. PCC6803|
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
to be added when available, antibody released in November 2013.
Left panel: from 0.15 to 5.17 µg of total protein from Synechocystis PCC6803 extracted with SDS-sample buffer and recombinant EF-G1 (right panel) were separated on 10 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5 % milk powder in TBS-T for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 7 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 2 minutes.
Courtesy of Yichen Zhang, Department of Biochemistry and Molecular Biology, University of Massachusetts, USA
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