GAPC1/2 | Glyceraldehyde-3-phosphate dehydrogenase
AS15 2894 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||37 kDa|
|Confirmed reactivity||Arabidopsis thaliana
|Predicted reactivity||Anthurium amnicola, Andrographis paniculata, Arachis ipaensis, Beta vulgaris, Brassica napus, Brassica olerace, Cajanus caja, Camelina sativa, Capsella rubella, Capsicum annuum, Carthamus tinctorius, Daucus carota, Elettaria cardamomum, Eleutherococcus senticosus, Eucalyptus grandis, Glycine max, Gymnadenia conopsea, Hordeum vulgare, Jatropha curcas, Mangifera indica, Malus domestica, Manihot esculenta, Medicago truncatula, Mikania micrantha, Nicotiana benthamiana, Oryza sativa, Phaseolus vulgaris, Prunus persica, Raphanus sativus, Rosmarinus officinalis, Salvia officinalis, Solanum lycopersicum, Spinacia oleracea, Tamarix hispida, Tarenaya hassleriana, Theobroma cacao, Triticum monococcum, Triticum aestivum, Vaccinium uliginosum, Vigna radiata, Vitis vinifera, Zostera marina|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
Use of this antibody as a loading control should be supported with specific experimental data.
To be added when available, antibody released in November 2016.
40 μg total proteins from Arabidopsis wt, and GAPC1-6xFlag, gapc1-1, gapac1-1/gapc2-1, and gapc1-1/gapc2-2 mutants, extracted and denatured as described by Larkin (2007), were separated on 12% SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with TBS-T buffer with 5% skimmed milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for overnight at 4°C with agitation in TBS-T with 2% skimmed milk. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (Goat anti-rabbit IgG horse radish peroxidase conjugated, from ZSGB-BIO, China) diluted to 1:0000 in for 1h at RT with agitation. The blot was washed as above and developed for 1 min with EasySee Western Blot Kit (TRANSTM, China). Exposure time was 30 seconds.
Courtesy of Dr. Songhu Wang, Chengdu Institute of Biology, Chinese Academy of Sciences, China
Courtesy of Dr. Pradeep Kachroo, University of Kentucky, USA
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