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GAPC1/2 | Glyceraldehyde-3-phosphate dehydrogenase

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AS15 2894 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

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AS15 2894

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product information
Background

Glyceraldehyde-3-phosphate dehydrogenase is an enzyme that catalyzes the first step in glycolysis by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. This enzyme is essential for the carbohydrate metabolism and the maintenance of cellular ATP levels. 

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana GAPC1 and GAPC2 proteinsUniProt: P25858, Q9FX54

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized
Quantity 50 µg
Reconstitution

For reconstitution add 50 µl of sterile water

Storage
The antibody may be stored at -20℃f or one year in its original formulation. Additionally, antibody may be stored at 2℃ to 8℃ for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.
Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications

Western Blot (WB)

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Additional information
application information
Recommended dilution

1:1000 (WB)

Expected | apparent MW 37 kDa
Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity
Anthurium amnicola, Andrographis paniculata, Arachis ipaensis, Beta vulgaris, Brassica napus, Brassica olerace, Cajanus caja, Camelina sativa, Capsella rubella, Capsicum annuum, Carthamus tinctorius, Daucus carota, Elettaria cardamomum, Eleutherococcus senticosus, Eucalyptus grandis, Glycine max, Gymnadenia conopsea, Hordeum vulgare, Jatropha curcas, Mangifera indica, Malus domestica, Manihot esculenta, Medicago truncatula, Mikania micrantha, Nicotiana benthamiana, Oryza sativa, Phaseolus vulgaris, Prunus persica, Raphanus sativus, Rosmarinus officinalis, Salvia officinalis, Solanum lycopersicum, Spinacia oleracea, Tamarix hispida, Tarenaya hassleriana, Theobroma cacao, Triticum monococcum, Triticum aestivum, Vaccinium uliginosum, Vigna radiata, Vitis vinifera, Zostera marina
Not reactive in

No confirmed exceptions from predicted reactivity known in the moment

Additional information

To be added when available

Selected references

To be added when available, antibody released in November 2016.


Application example


Western blot using anti-GAPC1/2 antibodyMutants used for application example
40 μg total proteins from Arabidopsis wt, and GAPC1-6xFlag, gapc1-1, gapac1-1/gapc2-1, and gapc1-1/gapc2-2 mutants, extracted and denatured as described by Larkin (2007), were separated on  12% SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with TBS-T buffer with 5% skimmed milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for overnight at 4°C  with agitation in TBS-T with 2% skimmed milk. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (Goat anti-rabbit IgG horse radish peroxidase conjugated, from ZSGB-BIO, China) diluted to 1:0000 in  for 1h at RT with agitation. The blot was washed as above and developed for 1 min with EasySee Western Blot Kit (TRANSTM, China). Exposure time was  30 seconds.

Courtesy of Dr. Songhu Wang, Chengdu Institute of Biology, Chinese Academy of Sciences, China



||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at support@agrisera.com