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product information
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| background |
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Goat anti-hen IgG, DyLight®488 Conjugate is a secondary antibody conjugated to DyLight®488, which binds to chicken immunoglobulins in immunological assays. The dye has a green color and absorbs at 493 nm and emits at 518 nm. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries. |
| antibody format |
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goat |
polyclonal |
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affinity purified goat IgG |
Lyophilized |
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| quantity |
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1 mg |
for reconstitution add 1,1 ml of sterile water. |
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| storage |
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Store undiluted liquid at 2-8°C. Shelf life is one year from a date of receipt. |
| related products |
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AS09 625 | Goat anti-hen IgG (H&L), DyLight®549 Conjugate |
| recommended dilution |
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1:20 – 1:2 000 (IHC), 1:50 – 1:5 000 (ICC) |
| additional information |
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Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. DyLight® 488 has a maximum absorbance at 493 nm; Emax = 518 nm
Fluor to protein ratio is: 1-5 Moles DyLight® 488 per Mole Antibody
No reactivity is observed to non-immunoglobulin hen serum proteins based in immunoelectrophoresis.
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application example 
BiP localization in 5 days old Arabidopsis thaliana roots. BiP signal shown in green, DAPI in blue. The material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Chicken anti-BiP primary antibody was diluted in 1: 1000 and DyLight®488 conjugated goat anti-chicken secondary antibody AS09 622 (green color) was diluted in 1: 1000. Co-staining with DAPI visualized nucleus (blue color). Scale bar – 10 µm. Courtesy Dr. Taras Pasternak, Freiburg University
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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