H3 | Histone H3 (chicken antibody)
AS15 2855 | clonality: polyclonal | host: chicken | reactivity: A. thaliana, C. reinhardtii, L. esculentum | cellular [compartment marker] of nucleoplasm
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|Recommended dilution||1 : 2500 (WB)|
|Expected | apparent MW||
15 | 17 kDa
|Confirmed reactivity||Arabidopsis thaliana, Chlamydomonas reinhardtii, Lycopersicum esculentum|
Arabidopsis thaliana, Brassica napus, Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Hordeum vulgare, Medicago sativa, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Pinus pinaster, Pisum sativum, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Triticum aestivum, Zea mays, Vicia faba, Vitis vinifera, Volvox sp.
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||to be added when available, antibody release in June 2015.|
From left to right: 2 wells of nuclear + cytoplasmic enriched protein, 2 wells of total proteins of Chlamydomonas reinhardtii protein saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (DayLight 800) 1:5000 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.
Courtesy of Dr. Betty Chung, University of Cambridge, United Kingdom
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