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L7/L12 | Ribosomal protein
AS08 331 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
- Product Info
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Immunogen: Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 200 µl Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunofluorescence (IF), Western blot (WB) Recommended dilution: 1: 10 (IF), 1 : 1000 (WB) Expected | apparent MW: 11.9 kDa (Chlamydomonas reinhardtii), 15.7 kDa (spinach)
- Reactivity
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Confirmed reactivity: Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 % Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example 
Total protein extracted freshly from Chlamydomonas reinhardtii denatured ay 90°C 5 min. were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blot was blocked with % milk or % BSA for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed chemiluminescent detection reagent, according to manufacture's recommendations. - Additional Information
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Additional information: Name of this antibody has been changed from L-30 | 50S ribosomal protein L30 to L7/L12 based on the following reference: Randolph-Anderson et al. (1989). Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved. J Mol Evol. 1989 Jul;29(1):68-88.
Additional information (application): Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.
- Background
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Background: Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96(5):1451-1463
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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Auroy Pascaline | 2022-03-11we used this antibody just good result for western blot (1/1000) and also immunofluorescence (diluted 10 times, 3h of incubation)
Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)