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Lhca2 | PSI type II chloropyll a/b-binding protein

300 €

AS01 006  |  clonality: polyclonal  |  host: rabbit  |  reactivity: monocots and dicots including A. thaliana, A. hypogaea, Colobanthus quitensis Kunt Bartl, H. vulgare, N. tabacum, O. sativa, P. sativum, P. vulgaris, P.banksiana, S. oleracea, Z. mays

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Item No:
AS01 006

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product information
background  

The light-harvesting protein Lhca2 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca2 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca2 forms a heterodimer (LHCI-680) with Lhca3 that associates with the PSI core close to PsaF and PsaK.

immunogen  

BSA-conjugated synthetic peptide derived from the Lhca2 protein of Arabidopsis thaliana (At3g61470). This sequence is highly conserved in Lhca2 proteins of angiosperms (monocots and dicots) and gymnosperms as well as in At1g19150. This gene codes for the very low expressed Lhca6 protein which also has been denoted as Lhca2*1.

antibody format  

rabbit

polyclonal,

total IgG in PBS pH 7.4,

lyophilized

quantity  

200 µl

- for reconstitution add 200 µl of sterile water

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB)

related products  

AS01 005 Lhca1 | PSI type I chloropyll a/b-binding protein

AS01 007 Lhca3 | PSI type III chlorophyll a/b-binding protein

AS01 008 Lhca4 | PSI type IV chlorophyll a/b-binding protein

AS05 082 Lhca5 | PSI type V chlorophyll a/b-binding protein

LHC  available antibodies against pigment-binding proteins

additional information  

A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073

application information
recommended dilution  

1 : 2000 - 1: 5000, detected with standard ECL (WB)

expected | apparent MW  

23 | 24 kDa for Arabidopsis thaliana

confirmed reactivity  

Arabidopsis thaliana, Arachis hypogaea, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Pinus banksiana (the higher of the two bands detected at 24 and 30 kDa is not considered to be specific to any Lhc protein), Spinacia oleracea, Zea mays

predicted reactivity  

angiosperms (monocots and dicots), gymnosperms

not reactive in  

no confirmed exceptions from predicted reactivity known in the moment

additional information  

The antibody will even recognize specifically one Lhca-type from Chlamydomonas reinhardtii

selected references  

Yamatani et al. (2013). NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll-protein complexes during leaf senescence. Plant J Feb 22.

Albus et al. (2012). LCAA, a novel factor required for Mg protoporphyrin monomethylester cyclase accumulation and feedback-control of aminolevulinic acid biosynthesis in tobacco. Plant Physiol. Oct 19.

 



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