Plant/Algal cell antibodies / Photosynthesis / LHC

product information

The light-harvesting protein Lhca2 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca2 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca2 forms a heterodimer (LHCI-680) with Lhca3 that associates with the PSI core close to PsaF and PsaK.

BSA-conjugated synthetic peptide derived from the Lhca2 protein of Arabidopsis thaliana (At3g61470). This sequence is highly conserved in Lhca2 proteins of angiosperms (monocots and dicots) and gymnosperms as well as in At1g19150. This gene codes for the very low expressed Lhca6 protein which also has been denoted as Lhca2*1.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS01 005 Lhca1 | PSI type I chloropyll a/b-binding protein

AS01 007 Lhca3 | PSI type III chlorophyll a/b-binding protein

AS01 008 Lhca4 | PSI type IV chlorophyll a/b-binding protein

AS05 082 Lhca5 | PSI type V chlorophyll a/b-binding protein

LHC  available antibodies against pigment-binding proteins

A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073

application information

1 : 2000 - 1: 5000, detected with standard ECL (WB)

23 | 24 kDa for Arabidopsis thaliana

Arabidopsis thaliana, Arachis hypogaea, Hordeum vulgare, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Pinus banksiana (the higher of the two bands detected at 24 and 30 kDa is not considered to be specific to any Lhc protein), Spinacia oleracea, Zea mays

angiosperms (monocots and dicots), gymnosperms

no confirmed exceptions from predicted reactivity known in the moment

The antibody will even recognize specifically one Lhca-type from Chlamydomonas reinhardtii

Yadavalli et al. (2012). Differential degradation of photosystem I subunits under iron deficiency in rice. J Plant Physiol. March 22.

Tanaka & Tanaka (2005) Effects of chlorophyllide a oxygenase in Arabidopsis thaliana. Photosynthesis Res 85: 327-340