MPK6 | Mitogen-activated protein kinase 6

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AS12 2633 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


13 st
Item No:
AS12 2633

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product information
Background MPK6 (Mitogen-activated protein kinase 6) is involved in oxidative stress-mediated signaling cascade, in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. Activated in response to touch, wounding, low temperature, low humidity, salt stress, hydrogen peroxide, ozone, ACC (an ethylene precursor), jasmonic acid (JA), mastoparan and UVC. Activated in response to elicitors: oligogalacturonides, hexameric chitin fragments, fungal xylanase, and the bacterial flagellin and harpin. Synonymes: MAP kinase 6, AtMPK6.

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MPK6 protein, UniProt: Q39026, TAIR: At2g43790

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS12 2107 | anti-MPK4 | Mitogen-activated protein kinase 4, chicken antibodies
AS11 1799 | anti-JA | jasmonic acid, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

45 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica napus, Gossypium mexicanum
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Wang and Auwerx (2017). Systems Phytohormone Responses to Mitochondrial Proteotoxic Stress. Mol Cell. 2017 Nov 2;68(3):540-551.e5. doi: 10.1016/j.molcel.2017.10.006.

application example

western blot using Agrisera anti-MPK6 antibodies

0.2g of 21 days old Arabidopsis thaliana leaf tssue C=Col, 6= mpk6 (T-DNA mutant), 3= mpk3 (T-DNA mutant) was homogenized with 250 μ l Buffer A( 50 mM Tris – HCl pH 7.5, 0.33 M sucrose, 5 mM EDTA, 150 mM NaCl , complete protease inhibitor cocktail). The crude extracts were subjected to centrifugation at 10 000 g for 10 min to pellet the insoluble material.   Material was extracted with  250 μl of Buffer A (50 mM Tris – HCl pH 7.5, 0.33 M sucrose, 5 mM EDTA, 150 mM NaCl, complete protease inhibitor cocktail). The crude extracts were subjected to centrifugation at 10 000 g for 10 min to pellet the insoluble material. Supernatant (120 μ L)+ 4XSDS sample buffer 40 μ L= 200 μ L samples ↓ 95 °C 5min 10 μ L were separated on 10 % SDS-PAGE  and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with skim milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody in TBS-T at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:75 000 in  for 1h at RT with agitation. The blot was washed as above and developed with ECL West Pico (Thermofisher/Pierce) according to the manufacturer's instructions. Exposure time was  seconds.

Courtesy of Chika Tateda, University of Chicago, USA

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