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NtcA | Global nitrogen regulator

361 €

AS12 1873 | clonality: polyclonal  |  host: rabbit  |  reactivity: cyanobacteria

PRODUCT INFORMATION IN PDF

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AS12 1873

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product information
Background

NtcA acts as a transcriptional activator of genes subjected to nitrogen control. 

Immunogen

KLH-conjugated synthetic peptide derived from known cyanobacterial NtcA protein sequences including P33779

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 200 µg
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products

collection of antibodies to other cyanobacterial proteins

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

25 kDa

Confirmed reactivity Synechocystis sp. PCC6803
Predicted reactivity marine Synechococcus strains, Anabaena sp., Gleobacter sp., Trichodesmium sp.
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references Ge at al. (2017). Translating Divergent Environmental Stresses into a Common Proteome Response through the Histidine Kinase 33 (Hik33) in a Model Cyanobacterium. Mol Cell Proteomics. 2017 Jul;16(7):1258-1274. doi: 10.1074/mcp.M116.068080.

application example

western blot detection of NtcA

5 µg of total protein from Synechocystis sp. PCC 6803  extracted with PEB  were separated on 4-12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 32  seconds.


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