PIF5 | Phytochrome interacting factor 5
AS12 2112 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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1 : 1000 with ECL (WB)
|Expected | apparent MW||
49.3 | 62 kDa
|Predicted reactivity||please inquire|
|Not reactive in||
|Additional information||For detection of PIF5 please use the most sensitive detection reagent.|
to be added when available, antibody released in July 2015
10 µg of total protein from 4 day old dark grown Arabidopsis thaliana seedligns extracted with hot buffer (Shen et al., Plant Cell. 2008;20(6):1586-602) were separated on 8 % SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with the blocking buffer from Protein Detector LumiGLO western blotting kit (KPL, Cat: 54-12-50) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for overnight at 4 degree with agitation. The antibody solution was decanted and the blot was for 3 times for 5 min in the washing buffer from the same kit at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, (AS09 602 from Agrisera) diluted to 1:25 000 in for 2h at RT with agitation. The blot was washed as above and developed for 5 min with ECL (femto from life technology) according to the manufacturer's instructions. Exposure time was 2 minute.
Higher % of a gel can separate better cross-reacting band and PIF5 protein.
Courtesy of Dr. Ling Zhu, University of Texas, USA
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