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SPS | sucrose phosphate synthase, maize

320 €

AS06 185   |  clonality: polyclonal  |  host: rabbit  |  reactivity: Z.mays  | cellular [compartment marker] of cytoplasm

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AS06 185

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product information
background  

SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.

immunogen  

Synthetic peptide derived from Zea mays SPS protein sequence (P31927).

antibody format  

rabbit

polyclonal

total IgG in PBS pH 7.4

lyophilized

quantity  

100 µl

- for reconstitution add 100 µl of sterile water

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB)

related products  

AS03 035A   SPS | sucrose phosphate synthase, global

for other antibodies to various maize proteins, please inquire.

additional information  

total IgG concentration is 3 µg/µl

application information
recommended dilution  

1 : 2 000 with standard ECL (WB)

expected | apparent MW  

120 | ~130 for Zea mays

confirmed reactivity  

Zea mays

predicted reactivity  

Zea mays and the monocots Saccharum officinarum, Triticum aestivum and Oryza sativa

not reactive in  

no confirmed exceptions from predicted reactivity known in the moment

additional information  

to be added when available

selected references  

Sparks et al. (2001) A Appl Biol 138: 33-45. Potential for manipulating carbon metabolism in wheat


application example

10 µg of total leaf protein from (1) A.thaliana, (3) Zea mays and (4) Hordeum vulgare extracted with PEB (AS08 300) as well as 10 µg cytosolic protein from (2) A.thaliana were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SPS (AS06 0185, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder.  Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (90s, high sensitivity).

 

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