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Plant/Algal cell antibodies
- Protein standards-quantitation
- Global antibodies
- Compartment markers
- Bioenergetics
- Carbohydrates
- Developmental biology
- DNA/RNA/cell cycle
- Environmental stress
- Fermentation
- Food proteins
- Hormones
- Mitochondria | Respiration
- Membrane transport system
- Nitrogen metabolism
- Photosynthesis
- Plant pathogens
- Toxins
- Tag antibodies
- Secondary antibodies/blocking
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Animal cell antibodies
- Bacterial, insect and fungal
- Carrier proteins
- Fish proteins
- Human proteins
- Immunoglobulins
- Neurosteroids/Neurobiology
- Secondary antibodies/blocking
- Secondary antibodies
SPS | sucrose phosphate synthase, maize
AS06 185 | clonality: polyclonal | host: rabbit | reactivity: Z.mays | cellular [compartment marker] of cytoplasm
| Info: | Product suggestions | Add review |
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| application information |
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| recommended dilution | 1 : 2 000 with standard ECL (WB) |
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| expected | apparent MW | 120 | ~130 for Zea mays |
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| confirmed reactivity | Zea mays |
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| predicted reactivity | Zea mays and the monocots Saccharum officinarum, Triticum aestivum and Oryza sativa |
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| not reactive in | no confirmed exceptions from predicted reactivity known in the moment |
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| additional information | to be added when available |
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| selected references | Sparks et al. (2001) A Appl Biol 138: 33-45. Potential for manipulating carbon metabolism in wheat |
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application example 10 µg of total leaf protein from (1) A.thaliana, (3) Zea mays and (4) Hordeum vulgare extracted with PEB (AS08 300) as well as 10 µg cytosolic protein from (2) A.thaliana were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SPS (AS06 0185, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (90s, high sensitivity). |
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||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com

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