Plant/Algal cell antibodies / Chlamydomonas reinhardtii

product information

Thioredoxin Reductase (TR, TrxR) is the only known enzyme (EC 1.8.1.9) which is reducing thoredoxin (Trx). Activity of thoredoxin is essential for growth and survival of the cell. There seem to be one isoform of NtrC protein in Arabidopsis which includes an N-terminal reductase domain and a C-terminal domain related to thioredoxin proteins. Arabidopsis and Chlmydomonas NtrC proteins are ca. 568 amino acids long, but include ca. 80 amino acid signal peptides, for mature protein   size of ca. 488 amino acids.

KLH-conjugated peptide derived from N-terminal domain of NtrC of Chlamydomonas reinhardtii A8HNQ7

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

Collection of antibodies to Chlamydomonas proteins

Peptide target chosen from the N-terminal domain, nearly fully conserved within 3-4 NtrA-related proteins from Arabidopsis thalina and with NtrC related proteins from Chlamydomonas reinhardtii and Synechococcus sp. 7942 and other cyanobacteria

application information

1 : 500 with standard ECL (WB)

55.3 kDa

Chlamydomonas reinhardtii

Chlamydia sp. Ostreococcus luminarius, Synechococcus sp.

Arabidopsis thaliana

antibody will react with long NtrC of Chlamydomonas reinhardtii proteins and shorter NtrA-type rpoteins, which would be distinguished by migration size.

to be added when available