Plant cytoplasm marker antibodies

Loading control: In biology there is no universal control. One has to determine a priori what does NOT change in one's test conditions and use that. The choice of appropriate housekeeping protein would depend upon the particular experiment and needs to be confirmed.

Other possible internal controls:

-total protein (express fmol target protein/ug total protein)
-dry mass
-total chlorophyll (express fmol target protein/ug chlorophyll)
-cells (express fmol target protein/cell)
-total nitrogen (express fmol target protein/fmol N)
-experiment specific ratios of particular proteins (ex. fmol PsbA/fmol RbcL)
For short term experiments Rubico protein makes a good loading control as its turnover is slow. However, Rubisco level do vary with nutrient levels, particularly nitrogen.

Possible antibodies to use: RbcL or RA (Rubisco activase) OEC223.

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2 | Plant Cell Compartment Antibody Marker Set | 5 antibodies

1514 €

SA000002 | clonality: polyclonal | host: rabbit | reactivity: higher plants and algae

2 | Plant Cell Compartment Antibody Marker Set | 10 antibodies

3028 €

SA000003 | clonality: polyclonal | host: rabbit | reactivity: higher plants, algae

cFBPase | cytosolic fructose-1,6-bisphosphatase (cytoplasm marker in photosynthetic tissues))

325 €

AS04 043 | clonality: polyclonal | host: rabbit | reactivity: A.thaliana, B.napus, P.silvestris, O.sativa, Z. mays | cellular [compartment marker] of cytoplasm

NAB1 | nucleic acid binding protein 1, Chlamydomonas

320 €

AS08 333 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii | cellular [compartment marker] of Chlamydomonas cytoplasm

RbcL | Rubisco large subunit, form I and form II (10 µl)

138 €

AS03 037-10 | clonality: polyclonal | host: rabbit | reactivity: [global antibody] for higher plants, lichens, algae, cyanobacteria, dinoflagellates, diatoms | cellular [compartment marker] of plastid stroma in higher plants and cytoplasm in cyanobacteria

RbcL | Rubisco large subunit, form I and form II (100 µl)

352 €

AS03 037 | clonality: polyclonal | host: rabbit | reactivity: [global antibody] for higher plants, lichens, algae, cyanobacteria, dinoflagellates, diatoms | cellular [compartment marker] of plastid stroma in higher plants and cytoplasm in cyanobacteria

SPS | sucrose phosphate synthase, maize

320 €

AS06 185 | clonality: polyclonal | host: rabbit | reactivity: Z.mays | cellular [compartment marker] of cytoplasm

UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker)

325 €

AS05 086 | clonality: polyclonal | host: rabbit | reactivity: A.thaliana, H.vulgare, O.sativa, P. glauca, Populus sp. and more | cellular [compartment marker] of cytoplasm