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NAB1 | nucleic acid binding protein 1, Chlamydomonas

320 €

AS08 333 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii | cellular [compartment marker] of Chlamydomonas cytoplasm

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AS08 333

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product information
background  

In Chlamydomonas reinhardtii NAB1 (nucleic acid binding protein 1) interacts in the cytosol with mRNAs coding for light-harvesting proteins of Photosystem II. NAB1 has structural similarities (cold-shock domain, CSD) to the FRGY2-protein from Xenopus laevis. According to immunolocalization studies NAB1 protein was exclusively found in cytosol, Mussgnug et al. (2005).

immunogen  

His-tagged recombinant NAB1 from Chlamydomonas reinhardtii (Q8GV23)
antibody format  

rabbit

polyclonal

serum

lyophilized
quantity  

100 µl

for reconstitution add 100 µl of sterile water.
storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications  

western blot (WB), immunoprecipitation (IP)
related products  

Collection of antibodies to Chlamydomonas proteins
additional information  

NAB1 can be used as a marker of cytoplasm in Chlamydomonas reinhardii.

application information
recommended dilution  

1: 10 000 standard ECL (WB)
expected | apparent MW  

26.5 kDa | 26 kDa (for Chlamydomonas reinhardtii)
confirmed reactivity  

Chlamydomonas reinhardtii
predicted reactivity  

Chlamydomonas reinhardtii
not reactive in  

no confirmed exceptions from predicted reactivity known in the moment
additional information  

to be added when available
selected references  

Almedia et al. (2012). Chlapsin, a chloroplastidial aspartic proteinase from the green algae Chlamydomonas reinhardtii. Planta Feb 19.

Blifernez et al. (2011) Protein arginine methylation modulates light-harvesting antenna translation in Chlamydomonas reinhardtii. Plant J. 65: 119-130.

Wobbe et al. (2009) Cysteine modification of a specific repressor protein controls the translational status of nucleus-encoded LHCII mRNAs in Chlamydomonas. Proc. Natl. Acad. Sci. USA 106: 13290-13295

Mussgnug et al. (2005) NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii. Plant Cell 17:3409-3421

application example

Western blot obtained using 1:10000 anti-NAB1 (AS08 333) and 1:160000 AP-conjugated secondary antibody on (1) recombinant NAB1 protein, (2) cytosolic protein of Chlamydomonas reinhardtii wild strain Cw10, (3) NAB1-deficient strain Stm3, and (4) Stm3 complemented with NAB1.

||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com