Plant/Algal cell antibodies / Compartment markers / Cytoplasm marker

product information

In Chlamydomonas reinhardtii NAB1 (nucleic acid binding protein 1) interacts in the cytosol with mRNAs coding for light-harvesting proteins of Photosystem II. NAB1 has structural similarities (cold-shock domain, CSD) to the FRGY2-protein from Xenopus laevis.

His-tagged recombinant NAB1 from Chlamydomonas reinhardtii (Q8GV23)

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunoprecipitation (IP)

Collection of antibodies to Chlamydomonas proteins

NAB1 can be used as a marker of cytoplasm in Chlamydomonas reinhardii.

application information

1: 10 000 standard ECL (WB)

26.5 kDa | 26 kDa (for Chlamydomonas reinhardtii)

Chlamydomonas reinhardtii

Chlamydomonas reinhardtii

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

Almedia et al. (2012). Chlapsin, a chloroplastidial aspartic proteinase from the green algae Chlamydomonas reinhardtii. Planta Feb 19.

Blifernez et al. (2011) Protein arginine methylation modulates light-harvesting antenna translation in Chlamydomonas reinhardtii. Plant J. 65: 119-130.

Wobbe et al. (2009) Cysteine modification of a specific repressor protein controls the translational status of nucleus-encoded LHCII mRNAs in Chlamydomonas. Proc. Natl. Acad. Sci. USA 106: 13290-13295

Mussgnug et al. (2005) NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii. Plant Cell 17:3409-3421

application example

Western blot obtained using 1:10000 anti-NAB1 (AS08 333) and 1:160000 AP-conjugated secondary antibody on (1) recombinant NAB1 protein, (2) cytosolic protein of Chlamydomonas reinhardtii wild strain Cw10, (3) NAB1-deficient strain Stm3, and (4) Stm3 complemented with NAB1.