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product information
|
| background |
|
Tubulin alpha (TUA) together with beta tubulin is making up microtubules.
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| immunogen |
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KLH-conjugated peptide derived from available tubulin alpha chain sequences including Arabidopsis thaliana tubulin alpha-1-chain P11139, alpha-2/alpha-4 chain P29510, alpha-5 chain P20363, alpha-6-chain P29511 |
| antibody format |
|
rabbit |
polyclonal |
|
serum |
lyophilized |
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| quantity |
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200 µl |
for reconstitution add 200 µl of sterile water |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB), immunofluorescence (IF) |
| related products |
|
AS10 681 | tubulin beta chain |
| additional information |
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Please, note that tubulin alpha is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to unable detection on a western blot, analyzed material must contain enough cells with dividing activity. |
application information
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| recommended dilution |
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1: 1000 with standard ECL (WB), 1: 500 (IF) |
| expected | apparent MW |
|
49 | 52 kDa (Arabidopsis thaliana) |
| confirmed reactivity |
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Arabidopsis thaliana, Hordeum vulgare, Zea mays
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| predicted reactivity |
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dicots including: Brassica napus, Glycine max, Pisum sativum, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Vitis vinifera, monocots including: Triticum aestivum, Oryza sativa, trees: Picea sitchensis, Populus trichocarpa, moss: Physcomitrella patens, algae: Chlorella vulgaris, Euglena gracilis, Micromonoas pusilla, Ostreococcus lucimarinus |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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Peptide used to elict this antibody is not present in tubulin beta. |
| selected references |
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to be added when available, antibody released in October 2011 |
application example western blot
5 µg of total protein from Arabidopsis thaliana (1), Hordeum vulgare (2), Zea mays (3) extracted with Agrisera PEB extraction buffer were separated on 4-12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602 ) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL Advance according to the manufacturers instructions (GE Health care). Exposure time was 120 seconds. immunolocalization 
Tubulin alpha localization in roots of Arabidopsis thaliana. Tubulin alpha (red), nucleus (DAPI white). Plant material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Primary antibodies: Agrisera anti-tubulin alpha 1: 500. Secondary antibody: goat anti-rabbit IgG Alexa conjugated (red color), dilution 1: 500. Scale bar – 10 µm. Courtesy Dr. Taras Pasternak, Freiburg University, Germany
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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