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product information
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| background |
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Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family are also expressed during seed development. |
| immunogen |
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recombinant Arabidopsis thaliana Hsp101/ClpB, N-terminal 145 amino acids P42730 |
| antibody format |
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hen |
polyclonal |
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total IgY in PBS pH 8.0+ 0.02% sodium azide |
liquid |
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| quantity |
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| storage |
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store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS07 253 | anti-HSP101 | ClpB heat shock protein, N-terminal AS08 290 | anti-HSP101 | nuclear/cytoplasmatic heat shock protein, rabbit antibody with reactivity to Zea mays collection of antibodies to plant heat shock proteins |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 1000 with standard ECL (WB) |
| expected | apparent MW |
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101 kDa |
| confirmed reactivity |
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Arabidopsis thaliana |
| predicted reactivity |
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dicots including: Glycine max, Nicotiana tabacum, Vitis vinifera |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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to be added when available |
application example .jpg)
2 µg of total protein from (1) Arabidopsis thaliana WT stressed at 22ºC, (2) Arabidopsis thaliana WT stressed at 38ºC for 1.5 hour followed by a transfer to 22° for 2 hours, (3) Arabidopsis thaliana Hsp101 null mutnat grown at 22°C, (4) Arabidopsis thaliana Hsp101 null mutant stressed at 38ºC for 1.5 hour followed by a transfer to 22ºC for 2 hours were separated on 8 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000 from Abcam).
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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