Plant/Algal cell antibodies / Membrane transport system / Endomembrane system

product information

SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1,
Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 Q9LM02

store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunolocalization (IL)

AS09 481 | anti-BiP2 | luminal-binding protein 2

antibodies to other proteins of endomembrane system

SMT1 is an integral membrane protein of ER (Boutte et al., 2009) while BiP is a membrane associated protein.

application information

1: 500 - 1:1 000 with ECL Advance or + (WB), 1: 50 - 1: 100 (IL)

38 kDa

Arabidopsis thaliana

dicots including: Glycine max, Gossypium mexicanum, Ricinus communis

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

Wang and Benning (2012). TGD4 involved in ER-to chloroplast lipid trafficking is a phosphatidic acid binding protein. Plant J. January 23 (ahead of print).

Boutté Y, Rosa M.S.F, Men S, Chow C.M, Ebine K, Gustavsson G, Johansson L, Ueda T, Moore I, Jürgens G, Grebe M (2009). Endocytosis Restricts Arabidopsis KNOLLE Syntaxin To The Cell Division Plane During Late Cytokinesis. EMBO J