NtcA | global nitrogen regulator

336 €

AS12 1873 | clonality: polyclonal | host: rabbit | reactivity: cyanobacteria

PRODUCT INFORMATION IN PDF

product information

background

NtcA acts as a transcriptional activator of genes subjected to nitrogen control.

immunogen

KLH-conjugated synthetic peptide derived from known cyanobacterial NtcA protein sequences including P33779

antibody format

rabbit

polyclonal

affinity purified serum

lyophilized

quantity

200 µg

for reconstitution add 100 µl, of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products

collection of antibodies to other cyanobacterial proteins

additional information

to be added when available

application information
recommended dilution		1 : 1000 with standard ECL (WB)
expected \| apparent MW		25 kDa
confirmed reactivity		Synechocystis sp. PCC6803
predicted reactivity		marine Synechococcus strains, Anabaena sp., Gleobacter sp., Trichodesmium sp.
not reactive in		no confirmed exceptions from predicted reactivity are currently known
additional information		to be added when available
selected references		to be added when available, antibody released in April 2012.

application example

western blot detection of NtcA

5 µg of total protein from Synechocystis sp. PCC 6803 extracted with PEB were separated on 4-12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 32 seconds.

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