Plant/Algal cell biology / Oryza sativa

product information

Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen. In plant cells catalase is found in peroxisomes. This enzyme is involved in photorespiration and symbiotic nitrogen fixation.

purified native catalase from Ricinus communis

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

ELISA (ELISA), western blot (WB)

AS08 374 | anti-KatG | catalase peroxidase (HPI), cyanobacterial

0.1 % sodium azide is added as preservative.

For antibody re-suspending information check the tube lable.

application information

1: 8000 (ELISA), 1: 2000 with standard ECL (WB)

57 | 55  kDa

Ricinus communis

dicots including: Arabidopsis thaliana,Gossypium mexicanum, Vitis vinifera, monocots including: Hordeum vulgare, Oryza sativa, trees: Populus jackii

no confirmed exceptions from predicted reactivity known in the moment

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

Maeshima et al. (1992). Tissue-Specific Isoforms of Catalase Subunits in Castor Bean Seedlings. Plant and Cell Physiology 33:225-232.

application example

SDS-PAGE of crude preparation of catalaze purified from Ricinus communis  endosperm (1) and western blot detection of  crude preparation of Ricinus communis endosperm proteins (2) . Proteins were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-catalase antibodies (AS09 501, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.