Plant/Algal cell antibodies / Oryza sativa

product information

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.

KLH-conjugated synthetic peptide conserved in known higher plant HSC70 proteins including three isoforms of Arabidopsis thaliana HSC70-1 (NP_ 001119156.1), HSC70-2 (NP_195869.1) and HSC70-3 (NP_187555.1) as well as heat shock inducible Hsp70 of Arabidopsis thaliana  AT3g12580/T2E22_110 and At1g16030 and AT3g12580/T2E22_110

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunoprecipitation (IP)

AS08 348 | anti-chloroplastic HSP70

AS08 347 | anti-mitochondrial HSP70

Immunoprecipitation protocol using Agrisera anti-Hsp70 cytosolic antibodies can be found here.

application information

1: 3000 with standard ECL on 5 µg of protein per well (WB), 2-3 µl/protein extract of concentration 3-5 mg/ml

70 kDa

Arabidopsis thaliana, Cucumis sativus, Hordeum vulgare, Silene vulgaris, Solanum lycopersicum,  Zea mays, algae: Desmodesmus subspicatus

dicots including Glycine max, Pisum sativum,Solanum lycopersicum and monocots including Oryza sativa, Triticum aestivum,; trees: Populus balsamifera, moss: Physcomitrella patens, algae including Chlamydomonas reinhardtii and Volvox sp.

no confirmed exceptions from predicted reactivity known in the moment

not available at the moment

Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.

Holding (2011). Pyrophosphate dependent fructose-6-phosphate 1-phosphotransferase induction and attenuation of Hsp gene expression during endosperm modification in Quality Protein Maize. Plant Physiol Dec. 8 (ahead of print).

Tukaj and Tukaj (2010). Distinct chemical contaminants induce the synthesis of Hsp70 proteins in green microalgae Desmodesmus subspicatus: Heat pretreatment increases cadmium resistance. Journal of Thermal Biology 35 (5):239-244.

application example

1µg of total protein from (1) Horderum vulgare  pre heat shock leaf extracted with PEB (AS08 300), (2) Horderum vulgare post heat shock  (2h 40ºC) leaf extracted with PEB (AS08 300), (3) Zea mays pre heat shock total protein leaf extracted with PEB (AS08 300), (4) Zea mays post heat shock (2h 40ºC) total protein leaf extracted with PEB (AS08 300) were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF (Milipore). Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-HSP70 antibody (AS08 371, 1:20 000, 1h) and secondary anti-rabbit (1:20 000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. All steps were performed at RT with agitation. Signal was detected with ECL Advance (GE Healthcare)