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product information
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| background |
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Serine/threonine protein kinase (EC=2.7.11.1), known as STN8 in Arabidopsis, and its Stl1 homologue in Chlamydomonas, are STN7/Stt7-like proteins that are not required for state transitions. The Arabidopsis mutants deficient in stn8 gene demonstrated highly reduced levels of the phosphorylated proteins of the photosystem II (Bonardi et al., 2005 Varionen et al., 2005). Alternative name: Protein STATE TRANSITION 8.
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| immunogen |
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KLH-conugated synthetic peptide (amino acids 425 - 438) specific for Arabidopsis thaliana STN8 serine/threonine proteinkinase At5g01920 |
| antibody format |
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| rabbit |
polyclonal |
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affinity purified serum |
lyophilized |
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| quantity |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS10 1611 | STN7 | serine/threonine-protein kinase STN7, chloroplastic
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| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 2000 with standard ECL (WB) |
| expected | apparent MW |
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54.9 kDa or 46 kDa on 6 M urea gel
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| confirmed reactivity |
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Arabidopsis thaliana |
| predicted reactivity |
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Oryza sativa |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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Stn8 is a nuclear encoded protein which is localized in chloroplast, hence posses a chloroplastic target peptides (cTP) at the beginning of the amino acid sequence which is cut off. Accroding to TargetP (program that predicts the length of the cTP:s) the length of cTP for Stn8 is 49 amino acids. STN8 portein mobility can be also affected by urea present in the gel. |
| selected references |
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to be added when available, antibody released in February 2012.
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Thylakoids 3ug of chlorophyll/lane from Arabidopsis thaliana (I will fill in here) were extracted according to Sirpiö et al (2011, Methods Mol Biol. 2011;775:19-30) and were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5%milk in TTBS for 1h at room temperature (RT) with slow agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 over night at 4C with slow agitation in 1 % milk TTBS . The antibody solution was decanted and the blot was rinsed briefly only once, then washed once for 15 min only in TBS-T at RT with vigorous agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated ) diluted to 1:10 000 in 1% milk in TTBS for 2h at +4 with slow agitation. The blot was washed as above and incubated for 5 min with ECL-solution according to the manufacturers instructions. Exposure time was 1min in this picture i sent you, but ok response was detectable already after 15 seconds. Courtesy Dr. Maija Holmström, University of Turku, Finland
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