Plant/Algal cell antibodies / Photosynthesis / LHC

product information

Lhcb4 (CP29) is one of the 3 minor chlrorophyll a/b-binding proteins associated with Photosystem II in plants and algae. Lhcb4 has been suggested to act in the regulation of the chl a excited state concentration of PSII because of its ability of sensing lumenal pH resulting in reversible phosphorylation. In Arabidopsis thaliana 2 genes code for two isoforms Lhcb4.1 and Lhcb4.2. A third isoform (Lhcb4.3, At2g40100), probably only present in dicots, has found to be differently regulated and therefore has been denoted as Lhcb8.

BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb4 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana (Lhcb4.1 At5g01530 and Lhcb4.2 At3g08940).

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS06 117 Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas

LHC  available antibodies against pigment-binding proteins

PSII  available antibodies against Photosystem II proteins

An overview about the different Lhc-protein types in plants can be found in Klimmek et al. (2006) Abundantly and rarely expressed Lhc protein genes exhibit distinct regulation patterns in plants. Plant Physiol 140: 793-804.

application information

1:7 000 (WB)

31.9 | 29 kDa for Arabidopsis thaliana

Arabidopsis thaliana, Pisum sativum, Phaseolus vulgaris, Zea mays

dicots including: Nicotiana tabaccum and Populus), monocots including: Oryza sativa, Hordeum vulgare, gymnosperms and microalgae Ostrecococcus tauri; the target sequence is only weakly conserved in Physcomitrella patens

Chlamydomonas reinhardtii (please use AS06 117 for this organism)

for image of detection please refer to Kusaba et al. (2007)

Rudowska et al. (2012). Chloroplast biogenesis - correlation between structure and function. BBA, available on line, March 2012.

Fristedt and Vener (2011). High Light Induced Disassembly of Photosystem II Supercomplexes in Arabidopsis Requires STN7-Dependent Phosphorylation of CP29. PLoS ONE.