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Lhcb4 | CP29 chlorophyll a/b binding protein of plant PSII

300 €

AS04 045  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, C. sativus, O. sativa, P. sativum, P. vulgaris, Z. mays

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AS04 045

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product information
background  

Lhcb4 (CP29) is one of the 3 minor chlrorophyll a/b-binding proteins associated with Photosystem II in plants and algae. Lhcb4 has been suggested to act in the regulation of the chl a excited state concentration of PSII because of its ability of sensing lumenal pH resulting in reversible phosphorylation. In Arabidopsis thaliana 2 genes code for two isoforms Lhcb4.1 and Lhcb4.2. A third isoform (Lhcb4.3, At2g40100), probably only present in dicots, has found to be differently regulated and therefore has been denoted as Lhcb8.

immunogen  

BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb4 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana (Lhcb4.1 At5g01530 and Lhcb4.2 At3g08940).

antibody format  

rabbit

polyclonal,

total IgG in PBS pH 7.4

lyophilized,

quantity  

200 µl

- for reconstitution add 200 µl of sterile water

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB)

related products  

AS06 117 Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas

LHC  available antibodies against pigment-binding proteins

PSII  available antibodies against Photosystem II proteins

additional information  

An overview about the different Lhc-protein types in plants can be found in Klimmek et al. (2006) Abundantly and rarely expressed Lhc protein genes exhibit distinct regulation patterns in plants. Plant Physiol 140: 793-804.

application information
recommended dilution  

1:7 000 (WB)

expected | apparent MW  

31.9 | 29 kDa for Arabidopsis thaliana

confirmed reactivity  

Arabidopsis thaliana, Cucumis sativus L. cv. Jihong no. 2, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Zea mays

predicted reactivity  

dicots including: Nicotiana tabaccum and Populus), monocots including:  Hordeum vulgare, gymnosperms and microalgae Ostrecococcus tauri; the target sequence is only weakly conserved in Physcomitrella patens

not reactive in  

Chlamydomonas reinhardtii (please use AS06 117 for this organism)

additional information  

for image of detection please refer to Kusaba et al. (2007)

selected references  

Yamatani et al. (2013). NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll-protein complexes during leaf senescence. Plant J Feb 22.

Rudowska et al. (2012). Chloroplast biogenesis - correlation between structure and function. BBA, available on line, March 2012.



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