Plant/Algal cell antibodies / Photosynthesis / PSII (Photosystem II)

product information

The PsbI protein, previously named the 4.8-kDa protein, is encoded by the plastome. PsbI is a universal component of PSII and is highly conserved (e.g. there is 71% amino acid identicality between the Arabidopsis and Synechocystis 6803 proteins). The protein contains 36 to 38 amino acids in most species, with molecular masses ranging between 4.1 and 4.5 kDa. Synonymes: PSII-I, PSII 4.8 kDa protein

KLH-conjugated synthetic peptide derived from Synechocystis sp. PCC 6803 PsbI sequence Q54697

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS06 158 | PsbI | small subunit I of PSII

to be added when available

application information

1: 5000 - 1: 10 000 with standard ECL West pico (WB)

4.3  kDa

Synechocystis sp. PCC 6803

cyanobacteria

no confirmed exceptions from predicted reactivity known in the moment

loads higher than 0.5  µg of chlorophyll per well are not recommended

Dobakova et. al  (2007). Role of the PsbI Protein in
Photosystem II Assembly and Repair in the Cyanobacterium Synechocystis sp. PCC 6803 Plant Physiol 145:1681-1691.

application example

Membrane fraction (0.5 µg chl), prepared from Synechocystis sp. PCC 6803, was separated on 12% SDS-PAGE and blotted in 2 hours at RT to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS, probed with anti-PsbI (1:10 000) antibody in TTBS overnight at 8ºC and secondary anti-rabbit in TTBS (1:10000,1h, room temperature). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.