Plant/Algal cell antibodies / Mitochondria | Respiration

product information

CAH3 - carbonic anhydrase is a zinc-containing metalloenzyme that catalyzes the reversible interconversion of CO2 and HCO3–. It plays an important role in many physiological functions that involve decarboxylation or carboxylation reactions, including both photosynthesis and respiration.
 

recombinant carbonic anhydrase of Chlamydomonas reinhardtii  A8J4Z8 after cleavage of a fusion protein

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

antibodies to other photosynthetic proteins

Collection of antibodies to Chlamydomonas proteins

this antibody recognizes native and recombinant carbonic anhydrase of Chlamydomonas reinhardtii

application information

1:2000 with standard ECL (WB)

33.4 | 29 kDa

Chlamydomonas reinhardtii

Chlamydomonas reinhardtii only

Arabidopsis thaliana, Oryza sativa

to be added when available

Shutova et al. (2008). The photosystem II-associated Cah3 in Chlamydomonas enhances the O2
evolution rate by proton removal. EMBO J. 27:782-791.

application example

(1) Chlamydomonas reinhardtii thylakoid preparation,

(2) overexoressed carbonic anhydrase

primary antibody used in 1: 2000 dilution. Secondary

antibodies anti-rabbit IgG (GE Healthcare)

used at 1: 10 000 with standard ECL