SMT1 | Sterol methyltransferase 1

424 €

AS07 266 | clonality: polyclonal: | host: rabbit | reactivity: Arabidopsis thaliana | marker antibody of integral ER membrane


-1 st
Item No:
AS07 266

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product information

SMT1 (EC= is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. The protein is highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 Q9LM02, At5g13710

Host Rabbit
Clonality Polyclonal
Purity Affinity purified IgG
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ul of sterile water.

store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunolocalization (IL)
Related products

AS09 481 | anti-BiP2 | luminal-binding protein 2

antibodies to other proteins of endomembrane system

Additional information

SMT1 is an integral membrane protein of ER (Boutte et al., 2009) while BiP is a membrane associated protein.

Endogenous SMT1 is expressed at very low levels in root epidermis and root cap as compare to cortex and endodermis and therefore this can contribute to SMT1 detection problems.

application information
Recommended dilution

1: 500 - 1:1 000 with ECL Advance or + (WB), 1: 50 - 1: 100 (IL)

Expected | apparent MW

38 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Amborella trichopoda, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium mexicanum, Medicago truncatula, Populus trichopocarpa, Prunus persica, Ricinus communis, Theobroma cacao, Vitis vinifera,

Not reactive in

Hordeum vulgare, Triticum aestivum, Withania somnifera

Additional information SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutté Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.
Selected references Yoshimoto et al. (2014). Quality control of plant peroxisomes in organ specific manner via autophagy. J cell Science, August 1, 2014, 127 (15).

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