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AS14 2766 |  clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii


65 st
Item No:
AS14 2766

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product information

LHCSR3 (Stress-related chlorophyll a/b binding protein 3) plays a role in an efficient enery dissipation process, called non-photochemical quenching (NPQ), in Chlamydomonas reinhardtii. 


KLH-conjugated synthetic peptide derived from LHCSR3 protein sequence from Chlamydomonas reinhardtii, UniProt:A8J431

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS14 2819 | anti-LhcSR1, rabbit antibodies

AS15 3081 | anti-LhcSR (Physcomitrella patens), rabbit antibodies

anti-LHC  available antibodies against pigment-binding proteins including Chlamydomonas reinhardtii

Collection of antibodies to Chlamydomonas proteins

Algal protein extraction buffer

Secondary antibodies

Additional information

to be added when available

application information
Recommended dilution

1: 1000 with standard ECL (WB)

Expected | apparent MW

28 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Chlamydomonas reinhardtii

Not reactive in Arabidopsis thaliana, Neochloris oleoabundans, Phaeodactylum tricornutum, Physcomitrella patens
Additional information

antibody is also recognizing recombinant LHCSR3

Selected references

to be added when available, antibody released in October 2014.

application example

western blot using anti-LHCSR3 antibodies

Follwoing samples: 0.1 µg of LhcSR3 IB + HisTag (1), 0.05 µg of LhcSR3 IB + HisTag (2), 5 µg of Chlamydomonas reinhardtii wild type (CC124) total protein extract of photoautotrophically grown cells in light intensity: 60 µE (3), 5 µg of Chlamydomonas reinhardtii wild type (CC124) total protein extract of photoautotrophically grown cells in high light intensity: 500 µE (4) were separated on 15% Tris-Glycine SDS PAGE and blotted overnight to PVDF using tank transfer. Blots were blocked with 5% BSA/milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions.

Courtesy Dr. Roberta Croce, Biophysics of Photosynthesis Dep. Physics and Astronomy Faculty of Sciences VU University Amsterdam, The Netherlands

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