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GPA1 | Guanine nucleotide-binding protein subunit alpha 1

265 €
Buy 2 items of this product for 198 €/each
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AS12 2370 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

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AS12 2370

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product information
Background GPA1 (G PROTEIN ALPHA-1) is involved as a modulator or transducer in various transmembrane signaling systems. Together with GCR1, may regulate the cell cycle via a signaling cascade  Promotes abscisic acid (ABA) responses in guard cells. But, together with GCR1 and GB1, acts as a negative regulator of ABA during seed germination and early seedling development. Involved in the blue light (BL) signaling. Alternative name: GP-alpha-1.
Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana GPA1, UniProt: P18064, TAIR: AT2G26300

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution

For reconstitution add 50 ĩl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

Antibodies to ABA and proteins involved in ABA metabolism

Antibodies to proteins involved in photomorphogenesis

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 4000 with ECL (WB)

Expected | apparent MW

49.3 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Brassica napus, Capsicum annuum , Cynara cardunculus var. scolymus, Eschscholzia californica, Glycine max, Gossypium hirsutum, Hordeum vulgare, Lupinus luteus, Medicago truncatula, Morus notabilis, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Populus trichocarpa, Ricinus communis , Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Theobroma cacao, Triticum aestivum, Zea mays, Zostera marina, Vitis vinifera
Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

The nature of a 37 kDa cross-reacting band is not known. 

Selected references

To be added when available, antibody released in April 2016. 


Application example

western blot using anti-GPA1 antibodies

25 µg of total protein from the indicated Arabidopsis thaliana wilde type and gpa1-3 mutant were extracted with extraction buffer ( 250 mM sucrose, 100 mM HEPES-KOH pH 7.5, 5% glycerol, 1mM Na2MoO4 x 2H2O, 25mM NaF, 10 mM EDTA, 1mM DTT, 0.5% Triton X-100, protease inhibitor cocktail) and denatured with SDS loading dye (50 mM Tris-HCl pH6.8, 100mM DTT, 2%SDS, 10% glycerol, 0.025% bromophenol blue) at 70°C for 2-5 min were separated on 10% SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with Tris-buffered saline containing 0.05% Tween-20 (TBS-T) and 5% skimmed milk powder for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody (anti-GPA1, AS12 2370) at a dilution of 1: 5 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 15 min in TBS-T with milk powder at RT with agitation. Blot was incubated in secondary antibody (Goat-Anti-Rabbit HRP conjugate, AS09 602) diluted 1:5000 for 2h at RT with agitation. The blot was washed as above with TBS-T without milk powder and then developed with Thermo Scientific SuperSignal West Substrate (pico and femto mixed 1:1), before exposure to a CEA RP NEW film for 10min.

Courtesy of Dr. Elena Petusching, Georg-August-University Goettingen, Germany

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