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H3 | Histone 3 core | (monoclonal)

361 €
AS16 3197 | clonality: monoclonal | host: mouse | reactivity: human, Solanum lycopersicum, Populus sp., Zea mays

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AS16 3197

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product information
Background

Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

Immunogen KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminus of the protein
Host Mouse
Clonality Monoclonal
Clone
Purity Protein A purified in PBS with 0.05 % sodium azide
Format
Quantity 50 ĩg
Reconstitution
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications chromatin immunoprecipiation (ChIP), western blot (WB)
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AS16 3181 | anti-H3K18me1 | Histone H3 (monomethyl Lys18), rabbit antibodies
AS16 3168 | anti-H3T3pK4me1 | Histone H3 (monomethyl Lys4, p Thr3), rabbit antibodies
AS16 3169 | anti-H3T3pK4me2 | Histone H3 (dimethyl Lys4, p Thr3), rabbit antibodies
AS16 3172 | anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodies

AS15 2855 | anti-H3 | histone H3, chicken polyclonal antibodies
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AS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710

collection of antibodies to epigenetics
Additional information Antibody has been purified using Protein A.

Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
application information
Recommended dilution 1-2 µg/IP (ChIP), 1: 1000 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity

human

Predicted reactivity Solanum lycopersicum, Populus sp., Zea mays
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information To be added when available
Selected references To be added when available, antibody released in February 2016.

application example

western blot using anti-H3core | (monoclonal) antibodies

Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively). The anti-H3 core antibody was diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.