PR-2 | Pathogenesis-related protein 2
AS12 2366 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Glycine max
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1 : 1000 with standard ECL (WB)
|Expected | apparent MW||
37.3 kDa (processing aa 1-30, mature peptide 34.1 kD)
Arabidopsis thaliana, Glycine max (roots)
|Predicted reactivity||Brassica juncea, Brassica oleracea, Citrus chinensis, Glycine max, Litchi chinensis, Manihot esculenta, Nicotiana tabacum|
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
Does not cross-react with other 1,3-beta glucosidases.
Kim et al. (2014). The Arabidopsis Immune Adaptor SRFR1 Interacts with TCP Transcription Factors that
10 µg of total protein from Arabidopsis thaliana mature leaves were extracted with 8M UREA and were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% Milk in TBS-T 0,05% for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in TBS-T/Milk 5% for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was seconds 60 seconds.
Courtesy of Louis-Valentin Meteignier, PhD student, University of Sherbrooke, Canada
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