PR-2 | Pathogenesis-related protein 2
AS12 2366 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Agostis stolonifera cv. ‘Penncross’, Glycine max
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|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
37.3 kDa (processing aa 1-30, mature peptide 34.1 kD)
|Confirmed reactivity||Agostis stolonifera cv. ‘Penncross’, Arabidopsis thaliana, Glycine max (roots)|
|Predicted reactivity||Brassica juncea, Brassica oleracea, Citrus chinensis, Glycine max, Litchi chinensis, Manihot esculenta, Nicotiana tabacum|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
Does not cross-react with other 1,3-beta glucosidases.
|Selected references||Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)
Kim et al. (2014). The Arabidopsis Immune Adaptor SRFR1 Interacts with TCP Transcription Factors thatRedundantly Contribute to Effector-Triggered Immunity. Plant J. 2014 Apr 1. doi: 10.1111/tpj.12527.
10 µg of total protein from Arabidopsis thaliana mature leaves were extracted with 8M UREA and were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% Milk in TBS-T 0,05% for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in TBS-T/Milk 5% for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was seconds 60 seconds.
Courtesy of Louis-Valentin Meteignier, PhD student, University of Sherbrooke, Canada
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