cAPX | Ascorbate peroxidase (cytosolic)

355 €

AS06 180  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A.  thaliana (immunohistochemistry only), A. toxicaria, D. salina, H.vulgare, P.sativum, S. lycopersicum, S. tuberosum, S. vulgaris, P. silvestris, . Z. mays


15 st
Item No:
AS06 180

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product information

Ascorbate peroxidase (APX) is the enzyme catalyzing the ascorbate-dependent reduction of hydrogen peroxide. Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in fruits and photosynthetic tissues. AA is found in every compartment of the plant cell including the apoplast.


KLH-conjugated peptide derived from N-terminal of Zea mays cytosolic APX Q41772

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunoprecipitation (IP), Immunolocalization (IL), Western blot (WB)
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AS08 368 | anti-APX | ascorbate peroxidase

Plant protein extraction buffer

Secondary antibodies

Additional information Total IgG concentration is 3 ĩg/ĩl.
application information
Recommended dilution 2 ĩg (IP), 1 : 500 (IL), 1 : 10 000 (WB)
Expected | apparent MW

28 kDa

Confirmed reactivity Arabidopsis thaliana (Immunohistochemistry only) Dunaliella salina, A. toxicaria, Hordeum vulgare, Pisum sativum, Silene vulgaris, Solanum lycopersicum, Solanum lycopersicum, Solanum tuberosum, Picea silvestris, Zea mays
Predicted reactivity Brassica. juncea, Citrus sinensis, Fragaria ananassa, Gossypium hirsutum, Nicotiana tabacum, Solanum tuberosum, Sorghum bicolor, Pinus pinaster, Vitis vinifera
Not reactive in

Glycine max

Additional information
Selected references Aroca et al. (2015). S-sulfhydration: a new post-translational modification in plant systems. Plant Physiology March 2015 pp.00009.2015.
Terrile et al. (2014). Nitric oxide-mediated cell death is triggered by chitosan in F. eumartii spores. Pest Manag Sci. 2014 Apr 25. doi: 10.1002/ps.3814.
Tsaniklidis et al. (2013). L-Ascorbic acid metabolism in parthenocarpic and seeded cherry tomatoes. Plant Growth Regul,DOI 10.1007/s10725-013-9845-0. (Solanum lycopersicum, immunolocalization)

application example


(A) , (B)- control antibody, anti-PIN1, (C,D) immunolocalization using anti-cAPX antibodies. 

Courtesy of Dr. Taras Pasternak


immunolocalization using anti-cAPX antibodies
immunolocalization in high expressing neurons

Dissociated rat hippocampal neurons 14 DIV Transfection of FLAG- and Nuclear Export Signal-tagged APEX2 (variant of cAPX; FLAG-APEX2-NES). Fixation: 4% formaldehyde for 15 min RT, 0.1% TritonX-100 permeabilization 20 min RT, 5% FCS blocking 1 hr RT. All in PBS. Primary antibody: 1:200 rabbit anti-cAPX, 4°C incubated for 24h, 1:1000 mouse anti-FLAG, RT 1h. 
Secondary antibodies: 1:1000 anti-rabbit Alexa488, RT 1 h, 1:1000 anti-mouse Alexa561, RT 1 h.

Courtesy of Dr. Tony Cijsouw, Biederer lab, Department of Neuroscience Tufts University School of Medicine, USA

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