3-nitroY | Nitrotyrosine
AS10 706-100 | clonality: monoclonal | host: mouse | reactivity: nitrotyrosine
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|Recommended dilution||The optimal working dilution should be determined by the investigator.|
|Expected | apparent MW|
|Confirmed reactivity||Dog, Human, Mouse, Rat|
|Predicted reactivity||Higher plants|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity
0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot using standard ECL.
|Selected references||Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:
Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.
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