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3-nitroY | Nitrotyrosine (100 ĩl)

383 €

AS10 706-100  | clonality: monoclonal  |  host: mouse  |  reactivity: nitrotyrosine

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AS10 706-100

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product information
Background

Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species.

Immunogen

BSA-conjugated 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid

Host Mouse
Clonality Monoclonal
Clone IgG2A
Purity
Format Liquid
Quantity 100 ĩl
Reconstitution
Storage

store at -20°C; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), ELISA (ELISA), immunohistochemistry (IHC), immunoprecipitation (IP)
Related products

AS10 706-25 | anti-nitrotyrosine mouse antibody, 25 µg

Secondary antibodies

Additional information

1 mg/ml of Protein G purified IgG2A in PBS pH 7.4,  0.09 % sodium azide, 50 % glycerol

application information
Recommended dilution

needs to be determined for each application

Expected | apparent MW
Confirmed reactivity

human, mouse, rat, dog

Predicted reactivity

higher plants

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity
with non-nitrated tyrosine. Not species specific.

0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot using standard ECL

 

Selected references Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:
Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.


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