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HSP70B | Stromal alfa-HSP70

345 €

AS06 175  clonality: polyclonal  |  host: rabbit  |  reactivity: Ch. reinhardtii, D. subspicatus, P. patens

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AS06 175

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product information
Background

HSP70B is a nuclear-encoded, chloroplast-targeted chaperone of the HSP70 family. It is the major HSP70 in the stroma of Chlamydomonas reinhardtii chloroplasts. It interacts with HSP90C, CGE1, CDJ2, and VIPP1.

Immunogen

mature HSP70B protein UniProt: A8HYV3, expressed with N- and C-terminal hexahistidine tags in E. coli, purified with Ni-NTA

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB) immunoprecipitation (IP)
Related products

collection of antibodies to other heat shock proteins

collection of antibodies to Chlamydomonas reinhardtii

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1:10 000 with standard ECL (WB)

Expected | apparent MW

71.9 kDa

Confirmed reactivity

Chlamydomonas reinhardtii, Desmodesmus subspicatus, Physcomitrella patens

Predicted reactivity

Dunaliella salina, cyanobacteria

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references

Diaz-Troya et al. (2011). Inhibition of protein synthesis by TOR inactivation revealed a conserved regulatory mechanism of the BiP chaperone in Chlamydomonas. Plant Physiol, in press.

Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).

Tukaj and Tukaj (2010). Distinct chemical contaminants induce the synthesis of Hsp70 proteins in green microalgae Desmodesmus subspicatus: Heat pretreatment increases cadmium resistance. Journal of Thermal Biology 35 (5):239-244.  



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