PIP2-1-7 | Plasma membrane aquaporin isoforms 1-7 (C-terminal)
AS12 2110 | clonality: polyclonal | host: rabbit | reactivity: L. sativa, P. sativum, S. lycopersicum, Z. mays
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1: 3000 (WB), (IP)
|Expected | apparent MW||
30.7 | 30 kDa (Zea mays)
|Confirmed reactivity||Lactuca sativa, Pisum sativum, Solanum lycopersicum, Zea mays|
Arabidopsis thaliana, Brassica oleracea, Cicer arietinum, Coffea arabica, Cucumis sativus, Fragaria chiloensis, Glycine max, Medicago trunculata, Mimosa pudica, Nicotiana tabacum, Olea europaea, Phaseolus vulgaris, Pisum sativum, Pyrus communis, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum urartu, Vitis vinifera,monocots: Hordeum vulgare, Oryza sativa, Triticum aestivum, trees: Picea mariana, Populus trichocarpa
|Not reactive in||Hordeum vulgare|
|Additional information||detection pattern consists of di and monomer of PIP2-7
to be added when available, antibody released in May 2012.
10 µg of total protein from Zea mays roots (1), Phaseolus vulgaris leaves (2) or roots (3) extracted with a mixture of 250 mM sorbitol, 50 mM Tris–HCl (pH 8), 2 mM EDTA, and protease inhibitors [1 mM phenylmethylsulfonyl Xuoride, 1 mg ml-1 each of leupeptin, aprotinin, antipain, chymostatin, and pepstatin were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% milk in TBS-T for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3.000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed four times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:30 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 60 seconds.
Courtesy of Dr. Ricardo Aroca, CSIC, Spain
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