Tic40 | Inner envelope membrane translocon complex protein (chloroplast)
AS010 709 | clonality: polyclonal | host: rabbit | reactivity: A.thaliana , N. tabacum, C. roseus, O. sativa, V. vinifera cellular [compartment marker] of chloroplast inner envelope membrane
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1 : 2500 with standard ECL (WB),
|Expected | apparent MW||
48 | 45 kDa (Arabidopsis thaliana)
Arabidosps thaliana, Catharantus roseus, Nicotiana tabacum, Oryza sativa, Vitis vinifera
dicots: Pisum sativum, Ricinus communis;monocots, trees: Picea sitchenis, Populus trichocarpa, moss: Physcomitrella patens
|Not reactive in||
to be added when available
|Selected references||Yang et al. (2016). OsCLT1, a CRT-like transporter 1, is required for glutathione homeostasis and arsenic tolerance in rice. New Phytol. 2016 Feb 25. doi: 10.1111/nph.13908
Yang et al. (2016). LIR1 regulates light-dependent attachment of LFNR to the thylakoid membrane in plants. Plant Cell. 2016 Mar 3. pii: tpc.01027.2015.
Román et al. (2015). Non-redundant contribution of the plastidial FAD8 ω-3 desaturase to glycerolipid unsaturation at different temperatures in Arabidopsis. Mol Plant. 2015 Jun 13. pii: S1674-2052(15)00267-1. doi: 10.1016/j.molp.2015.06.004.
Yin et al. (2014). The membrane proteome of stroma thylakoids from Arabidopsis thaliana studied by successive in-solution and in-gel digestion. Physiol Plant. 2014 Nov 17. doi: 10.1111/ppl.12308.
Wang et al. (2014). Maintenance of Chloroplast Structure and Function by Overexpression of the Rice MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE Gene Leads to Enhanced Salt Tolerance in Tobacco. Plant Physiol. 2014 May 19;165(3):1144-1155.
Brillouet et al. (2013).The tannosome is an organelle forming condensed tannins in the chlorophyllous organs of Tracheophyta. Ann Bot. Sep. 11. (Vitis vinifera, immunofluorescence)
10-15µg of chlorophyll from isolated total plant material (Arabidopsis thaliana), chloroplasts and thylakoids extracted with a buffer containing (25 mM Tricine-NaOH, pH 7.8, 330 mM sorbitol, 1 mM EDTA, 10 mM KCl, 0.15% [w/v] bovine serum albumin, 4 mM sodium ascorbate, and 7 mM L-Cys) were separated on 12 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 10% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 overnight at 4⁰C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:15 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 60 seconds with a ImageQuant system from GE Healthcare, exposure time was 60 seconds.
Courtesy of Dr. Rikard Fristedt VU University Amsterdam Faculty of Sciences Department of Physics and Astronomy Biophysics of Photosynthesis, The Netherlands
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