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LHCSR3

265 €
Buy 2 items of this product for 198 €/each
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AS14 2766  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

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AS14 2766

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product information
Background

LHCSR3 (Stress-related chlorophyll a/b binding protein 3) plays a role in an efficient enery dissipation process, called non-photochemical quenching (NPQ), in Chlamydomonas reinhardtii

Immunogen

KLH-conjugated synthetic peptide derived from LHCSR3 protein sequence from Chlamydomonas reinhardtii, UniProt:A8J431

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western Blot (WB)
Related products

AS14 2819 | anti-LhcSR1, rabbit antibodies

AS15 3081 | anti-LhcSR (Physcomitrella patens), rabbit antibodies

anti-LHC  available antibodies against pigment-binding proteins including Chlamydomonas reinhardtii

Collection of antibodies to Chlamydomonas proteins

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 1000 with standard ECL (WB)

Expected | apparent MW

28 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Chlamydomonas reinhardtii, Chlorella sp., Phaeodactylum tricornutum

Not reactive in
Arabidopsis thaliana, Neochloris oleoabundans, Physcomitrella patens
Additional information

Antibody is also recognizing recombinant LHCSR3

Selected references Wei et al. (2017). Light Intensity is Important for Hydrogen Production in NaHSO3-Treated Chlamydomonas reinhardtii. Plant Cell Physiol. 2017 Mar 1;58(3):451-457. doi: 10.1093/pcp/pcw216.
Garibay-Hernández et al. (2016). Membrane proteomic insights into the physiology and taxonomy of an oleaginous green microalga. Plant Physiol. 2016 Nov 8. pii: pp.01240.2016. [Epub ahead of print]
Haraldsdóttir (2016). Protection against UV rays and other desirable biological activity in Chlorella sp. and Phaeodactylum tricornutum.

Application example

western blot using anti-LHCSR3 antibodies

Follwoing samples: 0.1 µg of LhcSR3 IB + HisTag (1), 0.05 µg of LhcSR3 IB + HisTag (2), 5 µg of Chlamydomonas reinhardtii wild type (CC124) total protein extract of photoautotrophically grown cells in light intensity: 60 µE (3), 5 µg of Chlamydomonas reinhardtii wild type (CC124) total protein extract of photoautotrophically grown cells in high light intensity: 500 µE (4) were separated on 15% Tris-Glycine SDS PAGE and blotted overnight to PVDF using tank transfer. Blots were blocked with 5% BSA/milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions.

Courtesy Dr. Roberta Croce, Biophysics of Photosynthesis Dep. Physics and Astronomy Faculty of Sciences VU University Amsterdam, The Netherlands

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