CP43' | IsiA homolog of plant CP43 positive control/quantitation standard
AS10 111S | recombinant protein standard for quantitation
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Standard curve: 3 loads are recommended (2.5 and 10 μl).
Positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.
This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
|Expected | apparent MW||
27 kDa (slightly larger than native protein due to His-tag)
|Not reactive in|
Concentration: after adding 225 µl of milliQ water final concentration of the standard is 0.15 pmoles/µl
Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.
|Selected references||Fraser et al. (2013). Photophysiological and Photosynthetic Complex Changes during Iron Starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942.PLoS ONE 8(3): e59861. doi:10.1371/journal.pone.0059861
Ryan-Keogh et al. (2012). Iron deficiency in cyanobacteria causes monomerization of photosystem I trimers and reduces the capacity for state transitions and the effective absorption cross section of photosystem I in vivo. J. of Phycology, 1:145-154.
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