Plant/Algal cell antibodies / Photosynthesis / PSII (Photosystem II)

product information

CP43’ is encoded by the IsiA gene. This chlorophyll (Chl)-binding protein is induced under iron deficiency conditions in certain cyanobacterial strains. It acts as dissipater of light energy protecting photosystem II from excessive excitation under iron-deficient conditions

KLH-conjugated synthetic peptide nearly perfectly conserved across known IsiA/CP43 proteins including Synechocystis PCC sp. 6803 CP43' Q55274

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS06 110 | anti-PsbC (CP43) rabbit antibody

collection of antibodies to other PSII proteins

Peptide used to elicit this antibody is also perfectly or highly conserved in known Pcb chlorophyll a/b binding proteins from Prochlorococcus and similar proteins from other cyanobacteria. Peptide target is partially conserved in CP43/PsbC. CP43' and CP43 can be distinguished by their size.

application information

1: 500 with standard ECL (WB)

37 | 27 kDa (in a Novex gel system)

Synechococcus sp. PCC 7942, Synechocystis sp. PCC 6803

cyanobacteria

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

to be added when available

application example

5 µg of total protein from (1) Synechocystis sp. PCC 6803 extracted with PEB (AS08 300), (2) Synechocystis sp. PCC 6803 grown in low iron culture, extracted with PEB), were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:20 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

Cross-reacting band above 50 kDa is Rubisco.