PsaH | PSI-H subunit of photosystem I

345 €

AS06 105  |  clonality: polylclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana, Nicotiana tabaccum, Spinacia oleracea


31 st
Item No:
AS06 105

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product information

PsaH (PSI-H) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin). Psa-H has been suggested to be involved in regulation of state1-state2 transitions. In plants and algae Psa-H is nuclear encoded and imported post-translationally into the chloroplast where it inserts into the thylakoid membrane.


KLH-conjugated synthetic peptide derived from the protein sequence of Arabidopsis thaliana for PsaH1(At3g16240) and PsaH2 (At1g52230). This peptide sequence is quite conserved in some dicots but not in monocots.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS06 143 PsaH | PSI-H subunit of photosystem I, Chlamydomonas

PSI available antibodies to Photosystem I proteins

Photosynthesis available antibodies to photosynthetic proteins

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

10 | 10 for Arabidopsis thaliana

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabaccum, Spinacia oleracea
Predicted reactivity

Arachis hypogaea, Brassica rapa, Medicago truncatula, Nicotiana sylvestris, Nicotiana tabaccum, Populus trichocarpa, Ricinus communis, Spinacia oleracea

Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Wang et al. (2017). The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis.Mol Plant. 2017 Jan 9;10(1):183-196. doi: 10.1016/j.molp.2016.12.006.
Tiwari et al. (2016). Photodamage of iron–sulphur clusters in photosystem I induces non-photochemical energy dissipation. Nature Plants Article number: 16035 (2016) doi:10.1038/nplants.2016.35.

application example

2 µg of total leaf of Arabidopsis thaliana (1) and Hordeum vulgare (2) and cellular protein of Chlamydomonas reinhardtii (3) and Synechococcus PCC 7942 (4) isolated with PEB (AS08 300) were separated on 4-12% Nupage Bis-Tris gels in in MES running buffer (Invitrogen) at 200V for 35 minutes. Proteins were transferred for 80 minutes at 30V to a PVDF membrane pre-wetted in methanol and equilibrated in 1X transfer buffer. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) and probed with anti-PsaH (AS06 105, 1:1000) and secondary HRP-conjugated goat anti-rabbit antibody (1:50 000) for 1 hr in TBS-T containing 2% ECL Advance blocking reagent (GE Healthcare). Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signals was detected after 10 s using ECL Advance detection reagent (GE Healthcare) according to the manufacturers instructions and a CCD imager (FluorSMax, Bio-Rad).


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