PsaH | PSI-H subunit of photosystem I
AS06 105 | clonality: polylclonal | host: rabbit | reactivity: Arabidopsis thaliana, Nicotiana tabaccum, Spinacia oleracea
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1: 1000 with ECL system (WB)
|Expected | apparent MW||
10 | 10 for Arabidopsis thaliana
Arabidopsis thaliana, Nicotiana tabaccum, Spinacia oleracea
Arachis hypogaea, Brassica rapa, Medicago truncatula, Nicotiana sylvestris, Nicotiana tabaccum, Populus trichocarpa, Ricinus communis, Spinacia oleracea
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
to be added whan available
|Selected references||Wang et al. (2017). The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis.Mol Plant. 2017 Jan 9;10(1):183-196. doi: 10.1016/j.molp.2016.12.006.
Tiwari et al. (2016). Photodamage of iron–sulphur clusters in photosystem I induces non-photochemical energy dissipation. Nature Plants Article number: 16035 (2016) doi:10.1038/nplants.2016.35.
2 µg of total leaf of Arabidopsis thaliana (1) and Hordeum vulgare (2) and cellular protein of Chlamydomonas reinhardtii (3) and Synechococcus PCC 7942 (4) isolated with PEB (AS08 300) were separated on 4-12% Nupage Bis-Tris gels in in MES running buffer (Invitrogen) at 200V for 35 minutes. Proteins were transferred for 80 minutes at 30V to a PVDF membrane pre-wetted in methanol and equilibrated in 1X transfer buffer. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) and probed with anti-PsaH (AS06 105, 1:1000) and secondary HRP-conjugated goat anti-rabbit antibody (1:50 000, Abcam) for 1 hr in TBS-T containing 2% ECL Advance blocking reagent (GE Healthcare). Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signals was detected after 10 s using ECL Advance detection reagent (GE Healthcare) according to the manufacturers instructions and a CCD imager (FluorSMax, Bio-Rad).
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