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PsaN | PSI-N subunit of photosystem I

355 €

AS06 109  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana

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AS06 109

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product information
Background

Photosystem I (PSI) of chloroplasts is a multisubunit membrane-protein complex that catalyzes the electron transfer from the reduced plastocyanin (or cytochrome c6) in the thylakoid lumen to the oxidized ferredoxin (or flavodoxin) in the chloroplast stroma. PsaN is necessary for docking plastocyanin to the PSI complex. PSI-N is the only subunit located entirely on the lumenal side of PSI.

Immunogen

KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana PsaN protein sequence At5g64040

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩg
Reconstitution For reconstitution add 133 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

PSI antibody collection

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

9.7 kDa

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum
Predicted reactivity Phaseolus vulgaris, Oryza sativa, Populus balsamifera, Zea mays,Vitis vinifera
Not reactive in

Chlamydomonas reinhardtii, Synechococcus so. 7942

Additional information
Selected references Hansson et al. (2007). Knock-out of the chloroplast encoded PSI-J Subunit of Photosystem I in Nicotiana tabacum: PSI-J is required for efficient electron transfer and stable accumulation of photosystem I. FEBS J. 274: 1734-1746.


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