PsbI | PsbI | small subunit I of PSII (cyanobacterial)

345 €

AS10 692  |  clonality: polyclonal  |  host: rabbit  |  reactivity: cyanobacteria


18 st
Item No:
AS10 692

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product information

The PsbI protein, previously named the 4.8-kDa protein, is encoded by the plastome. PsbI is a universal component of PSII and is highly conserved (e.g. there is 71% amino acid identicality between the Arabidopsis and Synechocystis 6803 proteins). The protein contains 36 to 38 amino acids in most species, with molecular masses ranging between 4.1 and 4.5 kDa. Synonymes: PSII-I, PSII 4.8 kDa protein


KLH-conjugated synthetic peptide derived from Synechocystis sp. PCC 6803 PsbI sequence Q54697

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS06 158 | PsbI | small subunit I of PSII

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 5000 - 1: 10 000 with standard ECL West pico (WB)

Expected | apparent MW

4.3 kDa

Confirmed reactivity

Synechocystis sp. PCC 6803

Predicted reactivity


Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

loads higher than 0.5  µg of chlorophyll per well are not recommended

Selected references

Dobakova et. al  (2007). Role of the PsbI Protein in
Photosystem II Assembly and Repair in the Cyanobacterium Synechocystis sp. PCC 6803 Plant Physiol 145:1681-1691.

application example


Membrane fraction (0.5 µg chl), prepared from Synechocystis sp. PCC 6803, was separated on 12% SDS-PAGE and blotted in 2 hours at RT to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS, probed with anti-PsbI (1:10 000) antibody in TTBS overnight at 8ºC and secondary anti-rabbit in TTBS (1:10000,1h, room temperature). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.

Courtesy Dr. Roman Sobotka, Laboratory of Photosynthesis, Trebon,
Czech Republic

  western blot detection using anti-PsbI antibodies (cyanobacterial)

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