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PsbR | 10 kDa protein of PSII

345 €

AS05 059 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, N. tabacum, P. sativum, S. oleracea, T. aestivum

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Item No:
AS05 059

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product information
Background

PsbR protein is found in plant Photosystem II and anticipate to play a role in water oxidation, yet the physiological significance of PsbR has remained obscure.

Immunogen

KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana PsbR P27202, At1g79040

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

PSII antibody collection

Secondary antibodies

Additional information
application information
Recommended dilution

1:15 000 with standard ECL (WB)

Expected | apparent MW

14 | 10 kDa

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea, Triticum aestivum
Predicted reactivity

Brassica campestris, Solanum tuberosum, Vitis vinifera, Zea mays

Not reactive in

Chlamydomonas reinhardtii, Hordeum vulgare, Synechococcus sp. PCC 7942

Additional information
Selected references Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.
Shen et al. (2016). The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice. Rice (N Y). 2016 Dec;9(1):20. doi: 10.1186/s12284-016-0094-5. Epub 2016 May 10.
Albanese et al. (2016). Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy. Photosynth Res. 2016 Jan 9.
Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.
Ido et al. (2014). Cross-Linking Evidence for Multiple Interactions of the PsbP and PsbQ Proteins in a Higher Plant Photosystem II Supercomplex. J Biol Chem. 2014 Jul 18;289(29):20150-7. doi: 0.1074/jbc.M114.574822. Epub 2014 Jun 9.

Application example

2 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Horderum vulgare leaf), (3) Chlamydomonas reinhardtii total cell , (4) Synechococcus sp. 7942 total cell were all extracted with PEB (AS08 300) and separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 1 second.

 

western blot detection using anti-PsbR antibody

 


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