PsbW | Small subunit W of PSII
AS05 060 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Nicotiana tabacum, Spinacia oleracea
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1:15 000 with standard ECL (WB)
|Expected | apparent MW||
13.6 | 6.1 kDa
|Confirmed reactivity||Arabidopsis thaliana, Horderum vulgare, Nicotiana tabacum, Spinacia oleracea|
dicots including Vitis vinifera, monocots including Oryza sativa, Zea mays, moss Physcomitrella patens
|Not reactive in||
Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942
not available at the moment
|Selected references||Suorosa et al. (2006). PsbR, a missing link in the assembly of the oxygen-evolving complex of plant photosystem II. J. Biol. Chem. 1: 145-150.
Garcia-Cerdan et al. (2008). Antisense inhibition of the PsbX protein affects PSII integrity in the higher plant Arabidopsis thaliana. Plant Cell Physiol. 2: 191-202.
2 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Horderum vulgare leaf ), (3) Chlamydomonas reinhardtii total cell , (4) Synechococcus sp. 7942 total cell were all extracted with PEB (AS08 300) and separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 1 second.
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